Synergy between horseradish peroxidase HRP and edible gelatin: A simple way to remove phenols and dyes simultaneously from wastewater

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-02-23 DOI:10.1016/j.bej.2025.109692

Jianling Feng , Huadeng Li , Ke Zheng , Yanbin Zeng , Guanqun Xie , Xiaoxia Wang

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引用次数: 0

Abstract

Wastewater containing phenols or dyes is quite common. Given the potential harmful effects of such wastewater on both the environment and human health, there is a continuing demand for efficient, green, and cost-effective methods to remove these pollutants. In this study, edible gelatin was employed as an additive in conjunction with horseradish peroxidase (HRP) to purify wastewater containing phenols and dyes. Under optimized conditions, the combination of HRP and gelatin effectively purified various types of wastewater containing different phenols, achieving removal rates of up to 90 %. Furthermore, this strategy was successfully applied to the degradation of mixed wastewater containing dyes (methyl orange, methylene blue or/and rhodamine B) in the presence of phenol. High removal rates for both phenol and the dyes were also observed and the TOC removal rate reached as high as 94 %. Finally, a removal mechanism involving the HRP/gelatin system was proposed based on UV-Vis detection, particle size analysis, LC-MS, and TOC results. This green and efficient strategy for the removal of phenols and dyes from wastewater represents a valuable contribution to sustainable environmental remediation.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.