Construction of a NIR fluorescent probe for tracking mitochondrial HOCl during liver injury

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology Pub Date : 2025-02-25 DOI:10.1016/j.jphotobiol.2025.113136

Cailing Fan , Kaifu Ma , Ran Chen , Tianyu Zhang , Yonghao Song , Lei Liu , Weijie Chi , Qinxi Dong , Wei Shu , Chaoyuan Zeng

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引用次数: 0

Abstract

The inappropriate or excessive drug and alcohol consumption is a major cause of liver injuries. The development of a tool capable of visualizing the liver injury process with high precision, and in real time is particularly urgent. In this study, we designed and synthesized a novel HOCl-responsive fluorescent probe, PTZ-NS, for tracking mitochondrial HOCl during liver injury. PTZ-NS exhibits excellent sensitivity and selectivity for HOCl, with a detection limit of 158 nM. It can specifically target mitochondria, and successfully distinguishing between HepG2 and L-02 cells. The probe was also effective in tracking changes of endogenous HOCl levels in cells and zebrafish, and it was used to capture the increase in HOCl content during ferroptosis. Crucially, PTZ-NS was successfully employed to monitor HOCl during alcohol-induced liver disease (AID) and drug-induced liver injury (DILI) in mice liver tissue. Overall, PTZ-NS not only facilitate the real-time and precise observation of liver injury but also open up promising avenues for future disease prevention and research.

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肝损伤过程中线粒体HOCl荧光探针的构建

不适当或过量的药物和酒精消费是肝损伤的主要原因。开发一种能够对肝损伤过程进行高精度实时可视化的工具尤为迫切。在这项研究中，我们设计并合成了一种新的HOCl响应荧光探针PTZ-NS，用于跟踪肝损伤过程中线粒体HOCl。PTZ-NS对HOCl具有良好的灵敏度和选择性，检出限为158 nM。它可以特异性靶向线粒体，并成功区分HepG2和L-02细胞。该探针还可以有效地跟踪细胞和斑马鱼内源性HOCl水平的变化，并用于捕获铁下垂过程中HOCl含量的增加。关键是，PTZ-NS成功用于监测小鼠肝组织酒精性肝病（AID）和药物性肝损伤（DILI）期间HOCl的变化。总的来说，PTZ-NS不仅促进了肝损伤的实时和精确观察，而且为未来的疾病预防和研究开辟了有希望的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of photochemistry and photobiology. B, Biology 生物-生化与分子生物学

CiteScore

12.10

自引率

1.90%

发文量

161

审稿时长

37 days

期刊介绍： The Journal of Photochemistry and Photobiology B: Biology provides a forum for the publication of papers relating to the various aspects of photobiology, as well as a means for communication in this multidisciplinary field. The scope includes: - Bioluminescence - Chronobiology - DNA repair - Environmental photobiology - Nanotechnology in photobiology - Photocarcinogenesis - Photochemistry of biomolecules - Photodynamic therapy - Photomedicine - Photomorphogenesis - Photomovement - Photoreception - Photosensitization - Photosynthesis - Phototechnology - Spectroscopy of biological systems - UV and visible radiation effects and vision.