Single Nucleotide Polymorphism Highlighted via Heterogeneous Light-Induced Dissipative Structure

IF 9.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

ACS Sensors Pub Date : 2025-01-23 DOI:10.1021/acssensors.4c0211910.1021/acssensors.4c02119

Shuichi Toyouchi, Seiya Oomachi, Ryoma Hasegawa, Kota Hayashi, Yumiko Takagi, Mamoru Tamura, Shiho Tokonami* and Takuya Iida*,

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引用次数: 0

Abstract

The unique characteristics of biological structures depend on the behavior of DNA sequences confined in a microscale cell under environmental fluctuations and dissipation. Here, we report a prominent difference in fluorescence from dye-modified single-stranded DNA in a light-induced assembly of DNA-functionalized heterogeneous probe particles in a microwell of several microliters in volume. Strong optical forces from the Mie scattering of microparticles accelerated hybridization, and the photothermal effect from the localized surface plasmons in gold nanoparticles enhanced specificity to reduce the fluorescence intensity of dye-modified DNA to a few %, even in a one-base mismatched sequence, enabling us to clearly highlight the single nucleotide polymorphisms in DNA. Fluorescence intensity was positively correlated with complementary DNA concentrations ranging in several tens fg/μL after only 5 min of laser irradiation. Remarkably, a total amount of DNA in an optically assembled structure of heterogeneous probe particles was estimated between 2.36 ymol (2.36 × 10^–24 mol) and 2.36 amol (2.36 × 10^–18 mol) in the observed concentration range. These findings can promote an innovative production method of nanocomposite structures via biological molecules and biological sensing with simple strategies avoiding genetic amplification in a PCR-free manner.

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异质光致耗散结构凸显单核苷酸多态性

生物结构的独特特性取决于DNA序列在环境波动和耗散下被限制在微尺度细胞中的行为。在这里，我们报告了在几微升体积的微孔中光诱导的DNA功能化异质探针颗粒组装中，染料修饰单链DNA的荧光显著差异。微粒子Mie散射产生的强光学力加速了杂交，而金纳米粒子中局部表面等离子体产生的光热效应增强了特异性，即使在单碱基不匹配的序列中，也能将染料修饰DNA的荧光强度降低到几个百分点，使我们能够清楚地突出DNA中的单核苷酸多态性。激光照射5 min后，荧光强度与互补DNA浓度呈正相关，浓度范围在几十fg/μL。值得注意的是，在观察到的浓度范围内，非均质探针粒子光学组装结构中的DNA总量估计在2.36 ymol （2.36 × 10-24 mol）和2.36 amol （2.36 × 10-18 mol）之间。这些发现可以促进一种创新的生产方法，通过生物分子和生物传感，以简单的策略避免基因扩增，以无pcr的方式制备纳米复合结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.