Enzymatically Degradable Electrospun Nanogel Scaffolds Promoted in Vitro Proliferation of rADSC

IF 4.7 2区化学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Polymer Materials Pub Date : 2025-02-10 DOI:10.1021/acsapm.4c0406710.1021/acsapm.4c04067

Pengwen Li, Jinze Li, Hao Zhang, Kaiying Liang, Jiana Huang, Yuyang Liu, Huamin Jiang, Ting Song, Hongyun Zang and Yan Li*,

{"title":"Enzymatically Degradable Electrospun Nanogel Scaffolds Promoted in Vitro Proliferation of rADSC","authors":"Pengwen Li, Jinze Li, Hao Zhang, Kaiying Liang, Jiana Huang, Yuyang Liu, Huamin Jiang, Ting Song, Hongyun Zang and Yan Li*, ","doi":"10.1021/acsapm.4c0406710.1021/acsapm.4c04067","DOIUrl":null,"url":null,"abstract":"Scaffolds that mimic the extracellular matrix (ECM) can promote the proliferation of stem cells. Poly(N-isopropylacrylamide) (PNIPAM)/gelatin (PG) nanogel electrospun membranes were designed to replicate the hierarchical and dynamic nature of ECMs. First, PG nanogels with semi-interpenetrating network structures were synthesized by emulsion polymerization. Characterization using 1H NMR and FTIR spectroscopies confirmed the successful incorporation of gelatin into the PNIPAM-based network. The PG nanogels were spherical with a size of 184.1–231.9 nm, and they demonstrated a thermoresponsive behavior. The rehydrated nanofibers exhibited an average diameter of 663.6 ± 50.1 nm and a Young’s modulus of 1.3 ± 0.1 MPa at 37 °C. The membrane completely degraded within 10 min of collagenase treatment. Adipose-derived stem cells from rats exhibited good proliferation on the PG membranes, with cell proliferation in the PNI50 group being 1.47 times that in the tissue culture plate (TCP) group. The PG nanofibers showed potential for stem cell expansion.","PeriodicalId":7,"journal":{"name":"ACS Applied Polymer Materials","volume":"7 4","pages":"2665–2674 2665–2674"},"PeriodicalIF":4.7000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Polymer Materials","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsapm.4c04067","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Scaffolds that mimic the extracellular matrix (ECM) can promote the proliferation of stem cells. Poly(N-isopropylacrylamide) (PNIPAM)/gelatin (PG) nanogel electrospun membranes were designed to replicate the hierarchical and dynamic nature of ECMs. First, PG nanogels with semi-interpenetrating network structures were synthesized by emulsion polymerization. Characterization using ¹H NMR and FTIR spectroscopies confirmed the successful incorporation of gelatin into the PNIPAM-based network. The PG nanogels were spherical with a size of 184.1–231.9 nm, and they demonstrated a thermoresponsive behavior. The rehydrated nanofibers exhibited an average diameter of 663.6 ± 50.1 nm and a Young’s modulus of 1.3 ± 0.1 MPa at 37 °C. The membrane completely degraded within 10 min of collagenase treatment. Adipose-derived stem cells from rats exhibited good proliferation on the PG membranes, with cell proliferation in the PNI50 group being 1.47 times that in the tissue culture plate (TCP) group. The PG nanofibers showed potential for stem cell expansion.

Abstract Image

查看原文本刊更多论文

酶降解电纺丝纳米凝胶支架促进rADSC体外增殖

模拟细胞外基质（ECM）的支架可以促进干细胞的增殖。设计了聚n -异丙基丙烯酰胺(PNIPAM)/明胶（PG）纳米凝胶电纺丝膜，以复制ecm的层次性和动态性。首先，采用乳液聚合法制备了具有半互穿网状结构的PG纳米凝胶。利用1H NMR和FTIR光谱进行表征，证实了明胶成功地结合到pnipam网络中。制备的PG纳米凝胶为球形，尺寸为184.1 ~ 231.9 nm，具有热响应特性。在37℃时，复水合纳米纤维的平均直径为663.6±50.1 nm，杨氏模量为1.3±0.1 MPa。膜在胶原酶处理后10分钟内完全降解。大鼠脂肪干细胞在PG膜上表现出良好的增殖能力，PNI50组细胞增殖能力是组织培养板（TCP）组的1.47倍。PG纳米纤维显示出干细胞扩增的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Applied Polymer Materials Multiple-

CiteScore

7.20

自引率

6.00%

发文量

810

期刊介绍： ACS Applied Polymer Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics, and biology relevant to applications of polymers. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates fundamental knowledge in the areas of materials, engineering, physics, bioscience, polymer science and chemistry into important polymer applications. The journal is specifically interested in work that addresses relationships among structure, processing, morphology, chemistry, properties, and function as well as work that provide insights into mechanisms critical to the performance of the polymer for applications.