Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (Ficus carica Linn.).

IF 3.6 3区生物学 Q1 BIOLOGY

Biology-Basel Pub Date : 2025-02-11 DOI:10.3390/biology14020184

Junting Sun, Hadir Yishake, Ming Wang, Hao Zhang, Jie Yan

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Abstract

Figs are an edible and medicinal plant rich in polyphenols and flavonoids with unique pharmacological effects. However, the mechanism of flavonoid synthesis in figs is not clear. In this study, fig fruits of six varieties were collected for RNA sequencing and UPLC-MS data collection. The results showed that a total of 39 differential metabolites were identified by targeted metabolomics, and their contents were determined by UPLC-MS. The clustered heat map analysis showed that most of the differential metabolites were highly accumulated in BRD and FY. A total of 62 flavonoid biosynthesis pathway genes were identified by transcriptome analysis, and FcCHS, FcCHI, FcFLS, FcCYP, and FcDFR were the key genes identified for the accumulation of flavonoids and flavonols in the dark-colored varieties. In addition, a total of 1671 transcription factor genes, mainly MYBs, bHLHs, and AP2/ERFs, were identified. This study will enrich the transcriptomic data of figs and provide some help in resolving the synthesis mechanism of fig flavonoids.

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无花果是一种食用和药用植物，富含多酚和类黄酮，具有独特的药理作用。然而，无花果中黄酮类化合物的合成机制尚不清楚。本研究采集了 6 个品种的无花果果实，进行了 RNA 测序和 UPLC-MS 数据采集。结果表明，定向代谢组学共鉴定出39种差异代谢物，并通过UPLC-MS测定了其含量。聚类热图分析表明，大多数差异代谢物在BRD和FY中高度积累。通过转录组分析，共鉴定出62个黄酮类化合物生物合成途径基因，其中FcCHS、FcCHI、FcFLS、FcCYP和FcDFR是深色品种中黄酮类化合物和黄酮醇积累的关键基因。此外，共鉴定出1671个转录因子基因，主要是MYBs、bHLHs和AP2/ERFs。这项研究将丰富无花果的转录组数据，并为解决无花果类黄酮的合成机制提供一些帮助。

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来源期刊

Biology-Basel Biological Science-Biological Science

CiteScore

5.70

自引率

4.80%

发文量

1618

审稿时长

11 weeks

期刊介绍： Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.