The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows.

IF 2 2区 农林科学 Q2 VETERINARY SCIENCES
Liang Tian, Fen Su, Xueyi Zhu, Xingyue Zou
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Abstract

This study aimed to evaluate the influence of back-fat thickness (BF), at mating of sows, on autophagy in placenta and the potential mechanism. The sows were divided into two groups according to their BF at mating: BFI (15-20 mm, n = 14) and BFII (21-27 mm, n = 14) as the maternal obesity group. The placental samples used for investigating autophagic function and fatty acid profiles were obtained by vaginal delivery. Our results demonstrated that autophagy defects were observed in placenta from BFII sows along with altered circulating and placental fatty acid profiles. Indicative of impaired autophagy, reduced autophagic vesicles as well as LC3-positive puncta were linked to decreased mRNA or protein expression of autophagy-related genes, including ATG5, ATG7, Beclin1, ATG12, LC3, LAMP1 and LAMP2 in the placenta of BFII sows (p < 0.05). Meanwhile, we found reduced conversion of LC3-I to LC3-II and up-regulated protein content of p62 in the placenta from BFII group (p < 0.05). Furthermore, excessive back-fat was also associated with increased activation of AKT/mTOR signaling and decreased mRNA content of transcription factors regulating the autophagic pathway, including PPARα and PGC1α, but increased mRNA expression of NcoR1 in placenta. Together, these findings indicate that maternal obesity incites autophagy injury in pig term placenta, which may contribute to augmented placental lipid accumulation and therefore impaired placental function.

母亲肥胖对瘦种母猪胎盘自噬的影响。
本研究旨在探讨母猪配种时背膘厚度(BF)对胎盘自噬的影响及其可能机制。根据母猪配种时的体长分为两组,母肥组为BFI (15 ~ 20 mm, n = 14)和BFI (21 ~ 27 mm, n = 14)。用于研究自噬功能和脂肪酸谱的胎盘样本是通过阴道分娩获得的。我们的研究结果表明,在BFII母猪的胎盘中观察到自噬缺陷,并改变循环和胎盘脂肪酸谱。BFII母猪胎盘中自噬相关基因(ATG5、ATG7、Beclin1、ATG12、LC3、LAMP1和LAMP2) mRNA或蛋白表达减少与自噬囊泡减少和LC3阳性点相关,这表明自噬受损(p < 0.05)。同时,我们发现BFII组胎盘中LC3-I向LC3-II的转化降低,p62蛋白含量上调(p < 0.05)。此外,过多的背脂肪还与AKT/mTOR信号激活增加以及调节自噬途径的转录因子(包括PPARα和PGC1α) mRNA含量降低有关,但胎盘中NcoR1 mRNA表达增加。综上所述,这些发现表明,母亲肥胖会刺激猪足月胎盘的自噬损伤,这可能导致胎盘脂质积累增加,从而损害胎盘功能。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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