Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020-2023.

IF 2 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary Sciences Pub Date : 2025-01-30 DOI:10.3390/vetsci12020099

Hongyu Chen, Yi Qing, Lei Xu, Ling Zhu, Wenqi Yin, Shuwei Li, Shengyao Kuang, Yuancheng Zhou, Zhiwen Xu

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引用次数: 0

Abstract

Porcine parvovirus (PPV) is a non-enveloped, single-stranded linear DNA virus that induces reproductive disorders in sows, particularly abortions in primiparous sows. This study investigated the prevalence of PPV in the southwestern region and conducted molecular characterization of PPV strains. An epidemiological survey was conducted on 1534 aborted fetuses from the southwestern region between 2020 and 2023, revealing an abortion rate of 3.00% due to PPV2, with the highest rate of 3.77% in Sichuan. Additionally, 2973 blood samples from sows were tested using ELISA, showing a PPV2 antibody positivity rate of 73.03% to 90%. Through shotgun metagenomics, PPV2 SC2020 was identified in aborted fetal samples from a pig farm in Pengzhou, Sichuan. PCR sequencing analysis yielded seven PPV2 genomic sequences, and the phylogenetic analysis of eight PPV2 strains with thirty reference strains showed distinct evolutionary branches. The virus was successfully isolated from PPV2-positive samples, and the phylogenetic analysis of PPV2 SC2020 revealed ORF1 gene homology of 94.9% to 99.3% and the ORF2 gene homology of 93.1% to 98.0%, with 34 reference strains. Homologous recombination analysis indicated that SC2020 is a recombinant strain of HeB03 and S1.

查看原文本刊更多论文

2020-2023年西南地区猪细小病毒2型的流行及分子特征

猪细小病毒（PPV）是一种非包膜单链线性DNA病毒，可引起母猪生殖障碍，特别是初产母猪流产。本研究调查了西南地区PPV的流行情况，并对PPV菌株进行了分子鉴定。对2020 - 2023年西南地区1534例流产胎儿进行流行病学调查，PPV2流产率为3.00%，以四川地区最高，为3.77%。另外，对2973份母猪血液样本进行ELISA检测，PPV2抗体阳性率为73.03% ~ 90%。通过霰弹枪宏基因组学，在四川彭州某猪场流产胎儿样本中鉴定出PPV2 SC2020。PCR测序得到7个PPV2基因组序列，8个PPV2菌株与30个参考菌株的系统发育分析显示出不同的进化分支。从PPV2阳性样本中成功分离到该病毒，PPV2 SC2020的系统发育分析显示，ORF1基因同源性为94.9% ~ 99.3%，ORF2基因同源性为93.1% ~ 98.0%，共有34个参考菌株。同源重组分析表明，SC2020是HeB03和S1的重组菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.