Bao-Feng Wang, Ying-Ying Wang, Yun-Lan Yi, Ping-Ping Cao
{"title":"A novel approach for lymphatic organoid embedding: eosin pre-staining and agarose pre-embedding.","authors":"Bao-Feng Wang, Ying-Ying Wang, Yun-Lan Yi, Ping-Ping Cao","doi":"10.1080/21688370.2025.2472091","DOIUrl":null,"url":null,"abstract":"<p><p>Adenoid organoids, as the primary immune barrier of the airway, provide valuable models for studying lymphatic tissue function, but their histological processing remains challenging due to their fragile structure and lack of adhesion. Here, we introduce a novel approach that combines eosin pre-staining with agarose pre-embedding to enhance visibility and structural integrity during paraffin embedding. This method simplifies sectioning and improves the quality of hematoxylin and eosin (HE) and immunofluorescence (IF) staining, yielding clear and stable signals. By addressing key limitations in lymphatic organoid processing, this technique provides a reliable solution for histological and IF studies, facilitating future research on adenoid organoids.</p>","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2472091"},"PeriodicalIF":3.6000,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue Barriers","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21688370.2025.2472091","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Adenoid organoids, as the primary immune barrier of the airway, provide valuable models for studying lymphatic tissue function, but their histological processing remains challenging due to their fragile structure and lack of adhesion. Here, we introduce a novel approach that combines eosin pre-staining with agarose pre-embedding to enhance visibility and structural integrity during paraffin embedding. This method simplifies sectioning and improves the quality of hematoxylin and eosin (HE) and immunofluorescence (IF) staining, yielding clear and stable signals. By addressing key limitations in lymphatic organoid processing, this technique provides a reliable solution for histological and IF studies, facilitating future research on adenoid organoids.
期刊介绍:
Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.