Antibiofilm Effects of Plant Extracts Against Staphylococcus aureus.

Abstract

The global rise in antimicrobial resistance poses a significant threat to public health, necessitating alternative therapeutic options. One critical challenge is treating infections caused by biofilm-forming bacteria, which are notably resistant to conventional antibiotics. Staphylococcus aureus, including methicillin-resistant strains (MRSA), is a major pathogen in biofilm-related infections, complicating treatment and leading to chronic cases. Plant extracts have emerged as promising alternatives, offering new avenues for effective treatment. This study evaluated the antibacterial and antibiofilm activities of commercial extracts of Vitis vinifera L. (grape), Camellia sinensis L. (green tea), Olea europaea L. (olive), Quercus robur (oak), and Coffea arabica L. (coffee) against S. aureus strains from ATCC collections and clinical isolates. Preliminary screening using the disk diffusion test assessed the zones of inhibition, which was followed by minimum inhibitory concentration (MIC) determination via broth microdilution, with Quercus robur L. showing the best overall MIC results. The results obtained demonstrate the strong antibacterial activity of the extracts, with the MIC values ranging from 0.2 to 12.4 mg/mL. Using the XTT reduction assay, the extracts inhibited biofilm growth by 80-85% after 24 h of incubation, with Coffea arabica L. achieving interesting antibiofilm activities. These findings suggest that the investigated plant extracts hold potential as antimicrobial agents and biofilm inhibitors, offering an alternative approach to tackling antimicrobial resistance. Further research is needed to explore their potential applications in developing novel adjuvant therapies.