Quantitative levels of interferon gamma as a biomarker in the aqueous and serum samples of infectious and noninfectious uveitis patients.

IF 2.9 Q1 OPHTHALMOLOGY

Journal of Ophthalmic Inflammation and Infection Pub Date : 2025-02-26 DOI:10.1186/s12348-025-00461-1

Sahar Saad Eldeen Mohamed Shaban, Hend Mohammed Safwat, Abeer Mohammed Abdelmohymen, Donia Ahmed Hassan, Mona Nabeh Mansour, Hanan Said Mohammed, Nora Seliem, Shahinaz El Attar, Doaa Refaat Amin, Seham Kamal Ahmed Khirala, Aya Ahmed Ghamry, Mona Gamal El Din Al Anany, Shaymaa Fathy Mohammed

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Abstract

Background: To study the utility of interferon gamma (IFN-γ) as a differentiating biomarker by assessing the aqueous humour and serum of patients with infectious and noninfectious uveitis.

Methods: A total of 40 patients with acute uveitis were divided into 2 groups (18 patients with infectious uveitis and 22 patients with noninfectious uveitis). All the subjects underwent a full ophthalmological examination. Aqueous humour (AqH) and serum samples were collected from uveitis patients. The quantitative levels of IFN-γ in aqueous medium and serum were measured by means of an enzyme-linked immunosorbent assay (ELISA).

Results: The quantitative level of IFN-γ in the aqueous humour was significantly greater (87.5 ± 81) (pg/ml) in infectious uveitis patients than in noninfectious uveitis patients (37.3 ± 9.9) (pg/ml) (p value = 0.006). However, the serum IFN-γ level (pg/ml) did not significantly differ between these groups (p value = 0.279). Thus, the IFN-γ level in the aqueous humour can be used to discriminate between infectious and noninfectious uveitis with 33.3% sensitivity and 100% specificity.

Conclusion: Aqueous IFN-γ can be used as a biomarker for differentiating between infectious and noninfectious uveitis.

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干扰素γ作为生物标志物在感染性和非感染性葡萄膜炎患者的水和血清样本中的定量水平。

背景：研究干扰素γ (IFN-γ)作为鉴别生物标志物的效用，通过评估感染性和非感染性葡萄膜炎患者的房水和血清。方法：将40例急性葡萄膜炎患者分为2组（感染性葡萄膜炎18例，非感染性葡萄膜炎22例）。所有受试者都接受了全面的眼科检查。采集葡萄膜炎患者房水（AqH）和血清样本。采用酶联免疫吸附试验（ELISA）测定血清和水溶液中IFN-γ的定量水平。结果：感染性葡萄膜炎患者房水中IFN-γ的含量（87.5±81）（pg/ml）明显高于非感染性葡萄膜炎患者（37.3±9.9）（pg/ml）（p值= 0.006）。血清IFN-γ水平（pg/ml）各组间差异无统计学意义（p值= 0.279）。因此，房水中IFN-γ水平可用于区分感染性和非感染性葡萄膜炎，敏感性为33.3%，特异性为100%。结论：IFN-γ可作为鉴别感染性和非感染性葡萄膜炎的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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