Methodology for the Evaluation of Varietal Resistance to Haplaxius crudus, Vector of the Causal Agent of Lethal Wilt in Oil Palm in Colombia.

IF 2.7 2区 农林科学 Q1 ENTOMOLOGY
Insects Pub Date : 2025-02-11 DOI:10.3390/insects16020197
Ivette Johana Beltrán-Aldana, Gladys Alejandra Romero-Guerrero, Eloina Mesa-Fuquen, Anuar Morales-Rodriguez
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引用次数: 0

Abstract

Lethal Wilt is a limiting disease for oil palm cultivation in the eastern and central zones of Colombia. In the eastern zone, it caused the eradication of approximately 8700 ha of oil palm between 2010 and 2022, with economic losses of more than 185 million dollars. Studies conducted by Cenipalma reported that the pathogen causing this disease is Candidatus Liberibacter, which is possibly transmitted by Haplaxius crudus (Van Duzee). The adults feed on the foliage of the palms and move between them, spreading the pathogen in the plantation. A strategy to contribute to the management of LW is establishing cultivars resistant to the insect vector; however, no resistant cultivars or sources of resistance have been identified in the country's commercial cultivars or germplasm collections. Therefore, this work aimed to design and validate a methodology to characterize the oil palm genotypes Elaeis guineensis and Elaeis oleifera and interspecific OxG hybrids against adults of H. crudus, evaluating resistance through antixenosis and antibiosis to identify genotypes with possible sources of resistance. An arena with leaflets of the different genotypes in free-choice tests was used to assess antixenosis. For antibiosis, entomological sleeves were installed on the palm leaves, which were infested with adults of H. crudus from a breeding unit. The results of antixenosis and antibiosis in both the first phase (design) and the second phase (validation) indicated greater preference and survival for the genotypes of E. guineensis and lower preference and survival for the interspecific hybrids and E. oleifera. In the genotype E. guineensis, the average mortality was reached after 30 days, while in E. oleifera and the hybrids, it occurred between the third and fourth days. The results of this research provide a reproducible methodology for the evaluation of oil palm germplasms against H. crudus and sucking insects for the selection of sources of resistance for incorporation into breeding programs.

哥伦比亚油棕致命枯萎病病原媒介原hahaxius crudus抗性评价方法研究。
致命枯萎病是哥伦比亚东部和中部油棕种植的一种限制性疾病。在东部地区,2010年至2022年期间,它导致大约8700公顷的油棕被消灭,经济损失超过1.85亿美元。Cenipalma的研究报道,引起本病的病原体是Candidatus Liberibacter,可能是由haplxius crudus (Van Duzee)传播的。成虫以棕榈树的叶子为食,并在它们之间移动,在种植园中传播病原体。建立抗虫品种是促进LW管理的一种策略;然而,在该国的商业品种或种质资源收集中没有发现抗性品种或抗性来源。因此,本研究旨在设计并验证油棕基因型几内亚油棕、油棕油棕及种间杂交油棕对油棕成虫的抗性鉴定方法,通过抗异种性和抗生素性评估油棕基因型对油棕成虫的抗性,以确定油棕基因型可能的抗性来源。在自由选择试验中,使用带有不同基因型小叶的竞技场来评估抗xenosis。对于抗生素,在棕榈叶上安装昆虫学套,棕榈叶上有来自繁殖单位的成虫。第一阶段(设计阶段)和第二阶段(验证阶段)的抗xenosis和抗生素结果表明,豚鼠基因型的偏好和存活率较高,种间杂交和油鸡的偏好和存活率较低。几内亚伊蚊的平均死亡发生在30天后,而油鸡和杂交伊蚊的平均死亡发生在第3 ~ 4天。本研究结果为油棕种质资源对油棕和吸虫的抗性评价提供了一种可重复的方法,为选择抗源纳入育种计划提供了依据。
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来源期刊
Insects
Insects Agricultural and Biological Sciences-Insect Science
CiteScore
5.10
自引率
10.00%
发文量
1013
审稿时长
21.77 days
期刊介绍: Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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