Magnetic rod-shaped Mn-based MOF as a multi-functional and recyclable platform for dual-mode ratiometric-based nitrite detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-02-27 DOI:10.1007/s00604-025-07054-8

Sameera Sh. Mohammed Ameen, Faisal Algethami, Khalid M. Omer

{"title":"Magnetic rod-shaped Mn-based MOF as a multi-functional and recyclable platform for dual-mode ratiometric-based nitrite detection","authors":"Sameera Sh. Mohammed Ameen, Faisal Algethami, Khalid M. Omer","doi":"10.1007/s00604-025-07054-8","DOIUrl":null,"url":null,"abstract":"<div><p>The development is shown of rod-shaped manganese-based metal–organic frameworks (Mn-MOFs) as hot- and cold-adapted oxidase-like nanozymes, with strong magnetic properties. These Mn-MOFs enable highly sensitive detection of nitrite ions, utilizing both convenient colorimetric ratio analysis and a visual instrument-free-based approach compatible with smartphone-based detection. The Mn-MOF showed multi-functional activity, such as cold/hot-adapted and magnetic oxidase-like activity, catalyzing the oxidation of chromogenic substrates 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB). Mn-MOF shows high oxidase activity with <i>V</i>max of 1.39 × 10<sup>−8</sup> M/s and <i>K</i>m of 0.068 mM for TMB oxidation. Nitrite ions further react with oxTMB to form a yellow color via diazotization resulting in the ratiometric change in absorbance (A<sub>652</sub>/A<sub>461</sub>). The color ratio is also quantified through the naked eye and/or smartphone app by analyzing RGB values, providing a rapid, portable, and cost-effective method for on-site detection. When applying Mn-MOF for smartphone-based nitrite detection, it performs excellent detection, with a linear range of 5.0–55.0 µM and a limit of detection of 0.18 µM, superior to most of the oxidase nanozyme-based nitrite sensing platforms. The detection platforms develop sensing probes using a reusable nanozyme that enables highly sensitive and selective detection of nitrite, featuring a broad linear range and a low limit of detection.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07054-8","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The development is shown of rod-shaped manganese-based metal–organic frameworks (Mn-MOFs) as hot- and cold-adapted oxidase-like nanozymes, with strong magnetic properties. These Mn-MOFs enable highly sensitive detection of nitrite ions, utilizing both convenient colorimetric ratio analysis and a visual instrument-free-based approach compatible with smartphone-based detection. The Mn-MOF showed multi-functional activity, such as cold/hot-adapted and magnetic oxidase-like activity, catalyzing the oxidation of chromogenic substrates 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB). Mn-MOF shows high oxidase activity with Vmax of 1.39 × 10⁻⁸ M/s and Km of 0.068 mM for TMB oxidation. Nitrite ions further react with oxTMB to form a yellow color via diazotization resulting in the ratiometric change in absorbance (A₆₅₂/A₄₆₁). The color ratio is also quantified through the naked eye and/or smartphone app by analyzing RGB values, providing a rapid, portable, and cost-effective method for on-site detection. When applying Mn-MOF for smartphone-based nitrite detection, it performs excellent detection, with a linear range of 5.0–55.0 µM and a limit of detection of 0.18 µM, superior to most of the oxidase nanozyme-based nitrite sensing platforms. The detection platforms develop sensing probes using a reusable nanozyme that enables highly sensitive and selective detection of nitrite, featuring a broad linear range and a low limit of detection.

Graphical Abstract

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.