A Data-Driven Approach to Quantifying Immune States in Sepsis.

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Shan Li, Tengxiao Liang, Fangliang Xing, Shangshang Jiang
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Abstract

In sepsis, understanding the interplay among white blood cells, lymphocytes, and neutrophils is crucial for assessing the immune condition and optimizing treatment strategies. Blood samples were collected from 512 patients diagnosed with sepsis and 205 healthy controls, totaling 717 samples. Data visualization analysis and three-dimensional numerical fitting were performed to establish a mathematical model describing the relationships among white blood cells, lymphocytes, and neutrophils. Self-organizing feature map (SOFM) was employed to automatically cluster the sepsis sample data in the three-dimensional space represented by the model, yielding different immune states. Analysis revealed that white blood cell, lymphocyte, and neutrophil counts are constrained within a three-dimensional plane, as described by the equation: WBC = 1.098 × Neutrophils + 1.046 × Lymphocytes + 0.1645, yielding a prediction error (RMSE) of 1%. This equation is universally applicable to all samples despite differences in their spatial distributions. SOFM clustering identified nine distinct immune states within the sepsis patient population, representing different levels of immune status, oscillation periods, and recovery stages. The proposed mathematical model, represented by the equation above, reveals a basic constraint boundary on the immune cell populations in both sepsis patients and healthy controls. Furthermore, the SOFM clustering approach provides a comprehensive overview of the distinct immune states observed within this constraint boundary in sepsis patients. This study lays the foundation for future work on quantifying and categorizing the immune condition in sepsis, which may ultimately contribute to the development of more objective diagnostic and treatment strategies.

脓毒症患者免疫状态量化的数据驱动方法
在脓毒症中,了解白细胞、淋巴细胞和中性粒细胞之间的相互作用对于评估免疫状况和优化治疗策略至关重要。从512名诊断为败血症的患者和205名健康对照者中采集血液样本,共计717份样本。通过数据可视化分析和三维数值拟合,建立描述白细胞、淋巴细胞和中性粒细胞之间关系的数学模型。采用自组织特征图(SOFM)对模型所代表的三维空间中的脓毒症样本数据进行自动聚类,得到不同的免疫状态。分析显示,白细胞、淋巴细胞和中性粒细胞计数被限制在一个三维平面内,如公式所示:WBC = 1.098 ×中性粒细胞+ 1.046 ×淋巴细胞+ 0.1645,预测误差(RMSE)为1%。该方程普遍适用于所有样本,尽管它们在空间分布上存在差异。SOFM聚类在脓毒症患者群体中确定了九种不同的免疫状态,代表了不同的免疫状态水平、振荡周期和恢复阶段。所提出的数学模型,由上式表示,揭示了脓毒症患者和健康对照中免疫细胞群的基本约束边界。此外,SOFM聚类方法提供了脓毒症患者在该约束边界内观察到的不同免疫状态的全面概述。本研究为今后脓毒症免疫状况的量化和分类奠定了基础,最终可能有助于制定更客观的诊断和治疗策略。
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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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