Adrian J T Teo, Jianhui Gu, Alexander Govyadinov, Pavel Kornilovitch, Peiyun Wang, Serene Goh, Nguyen Truong Tung, Zhen Peng, Keith Koh, King Ho Holden Li
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引用次数: 0
Abstract
We developed a mass-producible microfluidic device capable of long-term observations of single bacilli and bulk bacteria culture interactions for subsequent antimicrobial resistance (AMR) studies. The device provides high consistency across separate devices due to its standardized manufacturing process unlike conventional microfluidic devices. Mycobacteria bovis BCG and M. smegmatis are trapped within the microfluidic device using minimal equipment and capillary-based techniques, acting as a surrogate model for the highly pathogenic bacteria M. tuberculosis. Individual bacilli and bulk bacteria aggregates were observed across a span of ten growth cycles, revealing bacteria growth morphologies alike those in past research. We accordingly propose that this chip would be appropriate for observations of AMR trials involving M. tuberculosis.
Biosensors-BaselBiochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍：
Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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