3,4 Dihydroxyphenylethanol May Inhibit Metastasis in HepG2 Cells by Influencing the Expression of miR-21 and Genes Associated with Metastasis.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mahdi Alaee, Gholamreza Shahsavari, Mohammad Yazdi, Maryam Hormozi
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引用次数: 0

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the lethal malignancies with a poor prognosis due to metastatic complications. Matrix metalloproteinases (MMPs) and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs), have an important role in metastasis. MicroRNA-21 (miR-21) is significantly overexpressed in nearly all types of human cancers, including HCC. Targeting miR-21 pharmacologically could be a promising therapeutic approach for HCC. 3,4-dihydroxyphenylethanol (DHPE), a phenolic phytochemical compound found in olive, has potent antioxidant and anticancer properties. This study aimed to investigate the effect of DHPE on the expression of miR-21 with genes associated with metastasis (MMP-2, MMP-9, TIMP-1, and TIMP-2) and their correlation with miR-21 in HepG2 cells.

Methods: This experimental study had four groups, including a control, and three groups of treatment with different concentrations of DHPE (50, 100, and 150 µM) for 24 hours. The expression levels of genes were determined by RT-qPCR.

Results: The results showed that the treatment of cells with DHPE significantly reduced the expression of miR-21, MMP-2, MMP-9, and TIMP-1 but increased TIMP-2 compared to the control group; additionally, there was a negative correlation between miR-21 and TIMP-2 but a positive correlation between miR-21 with MMP-2, MMP-9, and TIMP-1.

Conclusions: The results showed that DHPE, likely by reducing the expression of miR-21, can increase TIMP-2 and reduce MMP-2, MMP-9, and TIMP-1 gene expression and may play a role in inhibiting cell migration in HepG2 cells.

3,4二羟基苯乙醇可能通过影响miR-21和转移相关基因的表达来抑制HepG2细胞的转移。
背景:肝细胞癌(HCC)是一种致命的恶性肿瘤,由于转移并发症,预后较差。基质金属蛋白酶(MMPs)及其抑制剂组织金属蛋白酶抑制剂(TIMPs)在肿瘤转移中具有重要作用。MicroRNA-21 (miR-21)在几乎所有类型的人类癌症中都显着过表达,包括HCC。药物靶向miR-21可能是一种很有前途的HCC治疗方法。3,4-二羟基苯乙醇(DHPE)是一种在橄榄中发现的酚类植物化学化合物,具有有效的抗氧化和抗癌特性。本研究旨在探讨DHPE对HepG2细胞中miR-21与转移相关基因(MMP-2、MMP-9、TIMP-1和TIMP-2)表达的影响及其与miR-21的相关性。方法:本实验研究分为四组,包括对照组和三组不同浓度DHPE(50、100、150µM)处理24小时。RT-qPCR检测基因表达水平。结果:结果显示,与对照组相比,DHPE处理细胞可显著降低miR-21、MMP-2、MMP-9和TIMP-1的表达,但升高TIMP-2的表达;此外,miR-21与TIMP-2呈负相关,而miR-21与MMP-2、MMP-9和TIMP-1呈正相关。结论:结果表明,DHPE可能通过降低miR-21的表达,使HepG2细胞中TIMP-2升高,MMP-2、MMP-9、TIMP-1基因表达降低,可能起到抑制细胞迁移的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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