Profiling the Tumor Immune Microenvironment of HPV-Associated Base of Tongue Squamous Cell Carcinoma.

IF 2.7 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

OncoTargets and therapy Pub Date : 2025-02-20 eCollection Date: 2025-01-01 DOI:10.2147/OTT.S505376

Reham M Alahmadi, Maaweya Awadalla, Najat Marraiki, Mohammed Alswayyed, Hajar A Alshehri, Amjad Alsahli, Hatim A Khoja, Osamah T Khojah, Rawan M Alahmadi, Nada Farid, Bandar Alosaimi

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引用次数: 0

Abstract

Background: Base of tongue squamous cell carcinoma (BOTSCC) is a prevalent and aggressive form of oral cancer, often associated with poor patient outcomes. The tumor microenvironment (TME) of HPV-positive BOTSCC is critical in influencing cancer progression and treatment response.

Objective: This study aims to analyze the TME of HPV-positive BOTSCC by examining the expression of key genes involved in various biological processes.

Methods: We utilized the RT2 Profiler PCR Array to quantify the expression of 168 genes related to inflammation, immunity, oncogenesis, tumor suppression, apoptosis, and angiogenesis. Enrichment analysis of cancer hallmarks was performed on all upregulated genes. Additionally, we investigated the correlation between the expression levels of the ten most highly upregulated genes and survival prognosis in HPV-associated BOTSCC patients.

Results: Our analysis revealed dysregulation of 42 genes associated with tumor-immune interactions, with 20 genes upregulated and 22 downregulated. Furthermore, we identified 64 genes linked to cancer development, with 33 upregulated and 31 downregulated. High-risk HPV (hr-HPV) genotypes were found in 81% of patients, predominantly HPV-35 and HPV-16.

Conclusion: This study highlights the complexity of the HPV-positive BOTSCC TME, underscoring the need for further research into molecular pathways and immune interactions to identify new therapeutic targets for improved cancer treatment.

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舌鳞癌hpv相关基底的肿瘤免疫微环境分析。

背景：舌底鳞状细胞癌（BOTSCC）是一种常见的侵袭性口腔癌，通常与患者预后差有关。hpv阳性BOTSCC的肿瘤微环境（TME）是影响肿瘤进展和治疗反应的关键因素。目的：本研究旨在通过检测hpv阳性的BOTSCC参与各种生物学过程的关键基因的表达，分析其TME。方法：我们利用RT2 Profiler PCR阵列定量分析了168个与炎症、免疫、肿瘤发生、肿瘤抑制、细胞凋亡和血管生成相关的基因的表达。对所有上调的基因进行肿瘤标记富集分析。此外，我们研究了hpv相关的BOTSCC患者中10个高度上调基因的表达水平与生存预后之间的相关性。结果：我们的分析显示42个与肿瘤免疫相互作用相关的基因失调，其中20个基因上调，22个基因下调。此外，我们确定了64个与癌症发展相关的基因，其中33个上调，31个下调。高危HPV （hr-HPV）基因型在81%的患者中发现，主要是HPV-35和HPV-16。结论：本研究强调了hpv阳性BOTSCC TME的复杂性，强调了进一步研究分子途径和免疫相互作用以确定新的治疗靶点以改善癌症治疗的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

OncoTargets and therapy BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ONCOLOGY

CiteScore

9.70

自引率

0.00%

发文量

221

审稿时长

1 months

期刊介绍： OncoTargets and Therapy is an international, peer-reviewed journal focusing on molecular aspects of cancer research, that is, the molecular diagnosis of and targeted molecular or precision therapy for all types of cancer. The journal is characterized by the rapid reporting of high-quality original research, basic science, reviews and evaluations, expert opinion and commentary that shed novel insight on a cancer or cancer subtype. Specific topics covered by the journal include: -Novel therapeutic targets and innovative agents -Novel therapeutic regimens for improved benefit and/or decreased side effects -Early stage clinical trials Further considerations when submitting to OncoTargets and Therapy: -Studies containing in vivo animal model data will be considered favorably. -Tissue microarray analyses will not be considered except in cases where they are supported by comprehensive biological studies involving multiple cell lines. -Biomarker association studies will be considered only when validated by comprehensive in vitro data and analysis of human tissue samples. -Studies utilizing publicly available data (e.g. GWAS/TCGA/GEO etc.) should add to the body of knowledge about a specific disease or relevant phenotype and must be validated using the authors’ own data through replication in an independent sample set and functional follow-up. -Bioinformatics studies must be validated using the authors’ own data through replication in an independent sample set and functional follow-up. -Single nucleotide polymorphism (SNP) studies will not be considered.