Development of a Dispersive Liquid-Liquid Microextraction Method for Quantification of Volatile Compounds in Wines Using Gas Chromatography-Mass Spectrometry.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metabolites Pub Date : 2025-02-13 DOI:10.3390/metabo15020129
Dinesha Katugampala Appuhamilage, Rebecca E Jelley, Emma Sherman, Lisa I Pilkington, Farhana R Pinu, Bruno Fedrizzi
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引用次数: 0

Abstract

Background/Objectives: This study reports the development of a straightforward, efficient, and cost-effective dispersive liquid-liquid microextraction (DLLME) method for the gas chromatography-mass spectrometry (GC-MS) analysis of volatile compounds present in wine. Methods: Four critical parameters were optimised using a D-optimal design to maximise extraction outcomes of the targeted analytes from a 10 mL sample, while minimising interference from other compounds. The analytical characteristics of the method were assessed using 36 target compounds. Results: The method provided satisfactory linearity (correlation coefficients > 0.990), good repeatability for both for intra- and inter-day measurements (RSD < 10.3%), and suitable recoveries of target analytes from both model (83-110%) and real matrices (80-120%). The validated method was subsequently applied to analyse the aroma profile of 30 New Zealand Pinot noir (PN) wine samples. Conclusions: This study contributes to the advancement of analytical techniques available to both industry and researchers to explore the complex aroma profiles of wines.

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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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