Potential Biomarkers of Fatal Hypothermia Revealed by UHPLC-MS Metabolomics in Mice.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-02-11 DOI:10.3390/metabo15020116

Xin-Zhi Cao, Zhong-Wen Wu, Xing-Yu Ma, Wei-Liang Deng, Ding-Hao Chen, Jia-Li Liu, Jia-Hao Li, Hui Wang, Bao-Qing Pei, Dong Zhao, Qi Wang

{"title":"Potential Biomarkers of Fatal Hypothermia Revealed by UHPLC-MS Metabolomics in Mice.","authors":"Xin-Zhi Cao, Zhong-Wen Wu, Xing-Yu Ma, Wei-Liang Deng, Ding-Hao Chen, Jia-Li Liu, Jia-Hao Li, Hui Wang, Bao-Qing Pei, Dong Zhao, Qi Wang","doi":"10.3390/metabo15020116","DOIUrl":null,"url":null,"abstract":"Background: The postmortem diagnosis of fatal hypothermia presents a considerable challenge in forensic medicine. Metabolomics, a powerful tool reflecting comprehensive changes in endogenous metabolites, offers significant potential for exploring disease mechanisms and identifying diagnostic markers.Methods: In this study, we employed ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) to perform a non-targeted metabolomic analysis of liver, stomach, spleen, and musculus gastrocnemius tissues from mice subjected to fatal hypothermia.Result: A substantial number of differential metabolites were identified in each tissue: 1601 in the liver, 420 in the stomach, 732 in the spleen, and 668 in the gastrocnemius muscle. The most significantly altered metabolites were as follows: magnoflorine (liver, upregulated, ranked first in fold-change), gibberellic acid (stomach, downregulated, ranked first in fold-change), nitrofurantoin (spleen, upregulated, ranked first in fold-change), and isoreserpin (gastrocnemius muscle, downregulated, ranked first in fold-change). Glycerophospholipid metabolism exhibited notable enrichment in all tissues (spleen: second, liver: tenth, stomach: eleventh, gastrocnemius muscle: twenty-first), as did tryptophan metabolism (spleen: thirteenth, liver: eighth, stomach: third, gastrocnemius muscle: seventeenth).Conclusions: Our findings provide insights into the metabolic perturbations associated with fatal hypothermia in different tissues and lay a foundation for the identification of potential tissue biomarkers for forensic diagnosis.","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857787/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolites","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/metabo15020116","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: The postmortem diagnosis of fatal hypothermia presents a considerable challenge in forensic medicine. Metabolomics, a powerful tool reflecting comprehensive changes in endogenous metabolites, offers significant potential for exploring disease mechanisms and identifying diagnostic markers.

Methods: In this study, we employed ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) to perform a non-targeted metabolomic analysis of liver, stomach, spleen, and musculus gastrocnemius tissues from mice subjected to fatal hypothermia.

Result: A substantial number of differential metabolites were identified in each tissue: 1601 in the liver, 420 in the stomach, 732 in the spleen, and 668 in the gastrocnemius muscle. The most significantly altered metabolites were as follows: magnoflorine (liver, upregulated, ranked first in fold-change), gibberellic acid (stomach, downregulated, ranked first in fold-change), nitrofurantoin (spleen, upregulated, ranked first in fold-change), and isoreserpin (gastrocnemius muscle, downregulated, ranked first in fold-change). Glycerophospholipid metabolism exhibited notable enrichment in all tissues (spleen: second, liver: tenth, stomach: eleventh, gastrocnemius muscle: twenty-first), as did tryptophan metabolism (spleen: thirteenth, liver: eighth, stomach: third, gastrocnemius muscle: seventeenth).

Conclusions: Our findings provide insights into the metabolic perturbations associated with fatal hypothermia in different tissues and lay a foundation for the identification of potential tissue biomarkers for forensic diagnosis.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.