{"title":"Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber.","authors":"Ernest Nailevich Komissarov, Roderic Gilles Claret Diabankana, Inna Abdeeva, Daniel Mawuena Afordoanyi, Sergey Vladimirovich Gudkov, Ekaterina Mikhailovna Dvorianinova, Sergey Alexandrovich Bruskin, Alexey Alexandrovich Dmitriev, Shamil Zavdatovich Validov","doi":"10.3390/jof11020140","DOIUrl":null,"url":null,"abstract":"<p><p>The host specificity of <i>Fusarium oxysporum</i> (<i>Fox</i>) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (<i>mimp</i>), normally distributed on the accessory chromosomes. The pattern of <i>mimp</i> associated with effector genes has been used to predict candidate effector profiles which characterize <i>Fox</i> formae speciales. In this study, we demonstrate the pathogenicity of strains <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407 and <i>Fusarium oxysporum</i> f.sp. <i>radicis-cucumerinum</i> (<i>Forc</i>) V03-2g in a common host plant (cucumber) and compare their genomes. The <i>Forl</i> ZUM2407 genome lacks <i>SIX</i> genes and their homologs, in contrast to <i>Forc</i> V03-2g. We predicted the total number of <i>mimp</i> elements in the genome of <i>Forl</i> ZUM2407 to be three-fold less than that of <i>Forc</i> V03-2g (10 and 36 copies, respectively). The <i>mimp</i> distribution pattern in <i>Forl</i> ZUM2407 was completely different from that present in <i>Forc</i> V03-2g. Candidate effector profile analysis did not predict that <i>Forl</i> ZUM2407 was able to infect cucumber plants like <i>Forc</i> V03-2g. Therefore, we assume that <i>Forl</i> ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the <i>mimp</i>-based approach.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856433/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fungi","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/jof11020140","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The host specificity of Fusarium oxysporum (Fox) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (mimp), normally distributed on the accessory chromosomes. The pattern of mimp associated with effector genes has been used to predict candidate effector profiles which characterize Fox formae speciales. In this study, we demonstrate the pathogenicity of strains Fusarium oxysporum f.sp. radicis-lycopersici (Forl) ZUM2407 and Fusarium oxysporum f.sp. radicis-cucumerinum (Forc) V03-2g in a common host plant (cucumber) and compare their genomes. The Forl ZUM2407 genome lacks SIX genes and their homologs, in contrast to Forc V03-2g. We predicted the total number of mimp elements in the genome of Forl ZUM2407 to be three-fold less than that of Forc V03-2g (10 and 36 copies, respectively). The mimp distribution pattern in Forl ZUM2407 was completely different from that present in Forc V03-2g. Candidate effector profile analysis did not predict that Forl ZUM2407 was able to infect cucumber plants like Forc V03-2g. Therefore, we assume that Forl ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the mimp-based approach.
期刊介绍:
Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.