Molecular Insights into the Role of the MET30 Protein and Its WD40 Domain in Colletotrichum gloeosporioides Growth and Virulence.

Abstract

Colletotrichum gloeosporioides is a major phytopathogen responsible for anthracnose in Capsicum annuum (pepper) which leads to significant yield losses. At present, the molecular mechanism of C. gloeosporioides pathogenesis is not very clear. In this study, we focused on the MET30 protein and its key WD40 domain, with an emphasis on its role in the biological functions of C. gloeosporioides. Bioinformatics analysis revealed that the MET30 protein contains a conserved F-box domain and multiple WD40 repeats, which interact with other proteins to participate in various cellular processes, including nutrient acquisition, stress responses, and pathogenicity. Gene knockout and complementation experiments demonstrated that deleting the MET30 protein or its WD40 domain significantly reduced the rates of spore production and hyphal growth while increasing tolerance to environmental stresses such as high salinity and oxidative stress. Furthermore, pathogenicity assays revealed that the WD40 domain of the MET30 protein is crucial for regulating fungal pathogenicity, as mutants lacking WD40 domains presented increased virulence on pepper leaves. These findings suggest that the WD40 domain, in synergy with the MET30 protein, regulates the pathogenicity and stress response of C. gloeosporioides, provides new insights into the molecular mechanisms of anthracnose, and offers potential strategies for effective disease control.