Prognostic value of folate-associated gene expression in stage II colon cancer.

IF 2.7 3区 医学 Q3 ONCOLOGY
Donia Kaidi, Elisabeth Odin, Yvonne Wettergren, Elinor Bexe Lindskog
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引用次数: 0

Abstract

Purpose: Prognostic variability in stage II colon cancer underscores the need for better risk stratification. Analyzing folate-associated gene expression in stage II colon cancer could provide researchers and clinicians with deeper insights into tumor biology and potentially aid in identifying early prognostic and/or predictive biomarkers.

Methods: Patients with stage II colon cancer and recurrence (n = 48) were matched to patients with a 5 year recurrence-free follow-up (n = 133). Gene expression of ABCC3, AMT, FPGS, GGH, MFT, PCFT, RFC-1, and TYMS was analyzed in tumor tissue and matching colon mucosa using qPCR and evaluated in relation to time to recurrence (TTR), as well as to demographic and clinicopathological variables.

Results: Independent of other covariates, TYMS expression in tumors, pT4 stage, and emergency surgery were associated with TTR. There were significant differences in expression levels of all examined genes between tumor and mucosa. ABCC3, GGH, and RFC-1 expression levels differed in mucosa between microsatellite instability-high (MSI-H) compared to microsatellite stable/microsatellite instability-low (MSS/MSI-L) tumors, whereas tumoral expression of AMT, GGH, and TYMS differed between MSI-H and MSS/MSI-L tumors. Depending on tumor location, the expression of ABCC3, AMT, GGH, and RFC-1 in mucosa, as well as the tumoral expression of AMT, GGH, PCFT and RFC-1 differed.

Conclusion: Low tumoral expression of TYMS was associated with worse TTR, independent of MSI status, pT stage, and emergency surgery. The indication of a better outcome for patients with MSI-H status and high tumoral TYMS expression might be of particular interest in the stratification of patients for immunotherapy.

叶酸相关基因表达在II期结肠癌中的预后价值。
目的:II期结肠癌的预后变异性强调了更好的风险分层的必要性。分析II期结肠癌中叶酸相关基因表达可以为研究人员和临床医生提供对肿瘤生物学更深入的了解,并可能有助于确定早期预后和/或预测性生物标志物。方法:II期结肠癌复发患者(n = 48)与5年无复发随访患者(n = 133)相匹配。采用qPCR分析ABCC3、AMT、FPGS、GGH、MFT、PCFT、RFC-1和TYMS基因在肿瘤组织和匹配结肠黏膜中的表达,并评估其与复发时间(TTR)、人口学和临床病理变量的关系。结果:与其他协变量无关,TYMS在肿瘤中的表达、pT4分期和急诊手术与TTR相关。所有检测基因在肿瘤和粘膜间的表达水平均有显著差异。ABCC3、GGH和RFC-1在微卫星不稳定性高(MSI-H)和微卫星稳定/微卫星不稳定性低(MSS/MSI-L)肿瘤中的表达水平不同,而AMT、GGH和TYMS在MSI-H和MSS/MSI-L肿瘤中的表达不同。黏膜中ABCC3、AMT、GGH、RFC-1的表达以及AMT、GGH、PCFT、RFC-1的肿瘤表达因肿瘤部位不同而不同。结论:肿瘤中TYMS的低表达与较差的TTR相关,与MSI状态、pT分期和急诊手术无关。对于MSI-H状态和高肿瘤TYMS表达的患者来说,更好的结果可能是对免疫治疗患者分层的特别兴趣。
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来源期刊
CiteScore
4.00
自引率
2.80%
发文量
577
审稿时长
2 months
期刊介绍: The "Journal of Cancer Research and Clinical Oncology" publishes significant and up-to-date articles within the fields of experimental and clinical oncology. The journal, which is chiefly devoted to Original papers, also includes Reviews as well as Editorials and Guest editorials on current, controversial topics. The section Letters to the editors provides a forum for a rapid exchange of comments and information concerning previously published papers and topics of current interest. Meeting reports provide current information on the latest results presented at important congresses. The following fields are covered: carcinogenesis - etiology, mechanisms; molecular biology; recent developments in tumor therapy; general diagnosis; laboratory diagnosis; diagnostic and experimental pathology; oncologic surgery; and epidemiology.
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