Uliginosibacterium silvisoli sp. nov., Isolated from Subtropical Forest Soil in Hunan Province, China.

Abstract

A Gram negative bacterium, designated H3^T, was isolated from a forest soil sample collected in Zhangjiajie National Forest Park, Hunan Province, PR China. Cells of strain H3^T were yellow-pigmented, strictly aerobic, motile by means of a single polar flagellum. Growth occurred at 15-37 °C (optimum, 25-30 °C), pH 6.0-8.0 (optimum, 7.0) and 0-1.0% (w/v) NaCl (optimum 0%). Phylogenetic analysis based on 16S rRNA gene sequences and genome showed that strain H3^T was affiliated to the genus Uliginosibacterium within the family Rhodocyclaceae of the class Betaproteobacteria and shared the highest sequence similarities with U. sediminicola M1-21 ^T (96.3%). The DNA G + C content of the genomic DNA was 61.4%. The major fatty acids of strain H3^T were C_16:0, summed feature 3 (16:1 ω7c/16:1 ω6c) and C_12:0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipid, an unidentified aminolipid, three unidentified lipids, an unidentified glycolipid, and two uncharacterized phospholipids. The major respiratory quinone was ubiquinone-8. The dDDH and ANI values between strain H3^T and the type strains of the genus Uliginosibacterium were 19.2-20.7% and 71.4-76.0%, respectively. Based on the evidences in this study, strain H3^T represents a novel species of the genus Uliginosibacterium, for which the name Uliginosibacterium silvisoli sp. nov. is proposed. The type strain is H3^T (= MCCC 1K09133^T = KCTC 8564 ^T = JSACC 33374 ^T).