tRF-3a-Pro: A Transfer RNA-Derived Small RNA as a Novel Biomarker for Diagnosis of Hepatitis B Virus-Related Hepatocellular Carcinoma.

IF 5.9 1区生物学 Q2 CELL BIOLOGY

Cell Proliferation Pub Date : 2025-02-24 DOI:10.1111/cpr.70006

Jingyi Si, Yanting Zou, Yifan Gao, Jia Chen, Wei Jiang, Xizhong Shen, Changfeng Zhu, Qunyan Yao

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Abstract

Hepatocellular carcinoma (HCC) is one of the most challenging malignancies of the digestive system. Screening for novel biomarkers and therapeutic targets is a promising strategy to enhance HCC prognosis. Recently, liquid biopsy with circulating nucleic acids as the detection targets has attracted much attention in the field of early screening of tumours. However, the diagnostic value and biological functions of transfer RNA-derived small RNAs (tsRNAs) in serum, particularly in HCC, remains unknown. In this study, we characterised the expression profile of tsRNAs in hepatitis B virus (HBV)-related HCC, and confirmed the diagnostic potential of serum tRF-3a-Pro. On this basis, we established a diagnostic model that integrates tRF-3a-Pro with the classic HCC biomarker alpha-fetoprotein (AFP) through logistic regression analysis. Besides, both in vivo and in vitro experiments demonstrated that tRF-3a-Pro, a highly expressed tsRNA, promotes HCC cell proliferation. These findings suggested that tRF-3a-Pro could serve as a novel biomarker for HBV-related HCC.

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tRF-3a-Pro：一种转移RNA衍生的小RNA作为诊断乙型肝炎病毒相关肝细胞癌的新生物标志物

肝细胞癌（HCC）是消化系统最具挑战性的恶性肿瘤之一。筛选新的生物标志物和治疗靶点是改善HCC预后的一种有希望的策略。近年来，以循环核酸为检测靶点的液体活检在肿瘤早期筛查领域备受关注。然而，血清中转移rna衍生的小rna （tsRNAs）的诊断价值和生物学功能，特别是在HCC中的诊断价值和生物学功能尚不清楚。在这项研究中，我们描述了tsRNAs在乙型肝炎病毒（HBV）相关的HCC中的表达谱，并证实了血清tRF-3a-Pro的诊断潜力。在此基础上，我们通过logistic回归分析，建立了将tRF-3a-Pro与HCC经典生物标志物甲胎蛋白（AFP）相结合的诊断模型。此外，体内和体外实验均表明，高表达的tsRNA tRF-3a-Pro可促进HCC细胞增殖。这些发现表明，tRF-3a-Pro可以作为hbv相关HCC的一种新的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Proliferation 生物-细胞生物学

CiteScore

14.80

自引率

2.40%

发文量

198

审稿时长

1 months

期刊介绍： Cell Proliferation Focus: Devoted to studies into all aspects of cell proliferation and differentiation. Covers normal and abnormal states. Explores control systems and mechanisms at various levels: inter- and intracellular, molecular, and genetic. Investigates modification by and interactions with chemical and physical agents. Includes mathematical modeling and the development of new techniques. Publication Content: Original research papers Invited review articles Book reviews Letters commenting on previously published papers and/or topics of general interest By organizing the information in this manner, readers can quickly grasp the scope, focus, and publication content of Cell Proliferation.