Enhanced Bladder Regeneration with Adipose-Derived Stem Cell-Seeded Silk Fibroin Scaffolds: A Comparative Analysis.

IF 3.4 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY
Hanan Hendawy, Ahmed Farag, Asmaa Elhaieg, Elsayed Metwllay, Kazumi Shimada, Ahmed Elfadadny, Ryou Tanaka
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Abstract

Effective bladder reconstruction remains a significant challenge in urology, particularly for conditions requiring partial or complete bladder replacement. In this study, the efficacy is evaluated of two types of scaffolds, silk fibroin (SF) and adipose-derived stem cells (ADSCs-SF), in promoting bladder regeneration and their associated outcomes. A rat model was used to compare the surgical outcomes and morphological recovery of bladder tissues implanted with SF and ADSCs-SF scaffolds. Post-operative recovery, including voiding ability and complication rates, was assessed. The morphological and histological changes of the regenerated bladder tissue were evaluated at multiple time points (2, 4, 8, and 12 weeks) using gross tissue analysis, histometric assessments, and immunohistochemical staining. Both scaffold types demonstrated successful integration into the bladder wall with no significant differences in body weight or voiding issues. The SF scaffold group exhibited graft shrinkage and a 41.6% incidence of bladder calculus formation. In contrast, the ADSCs-SF scaffold facilitated superior morphological restoration, with bladder tissue progressively adopting a more normal shape and no incidence of bladder calculus. Histological analysis revealed that the ADSCs-SF scaffold significantly promoted the regeneration of a more organized urothelium layer and smooth muscle tissue. It also resulted in higher vessel density and reduced infiltration of inflammatory cells when compared to the SF scaffold alone. Additionally, the ADSCs-SF group exhibited enhanced expression of key markers, including uroplakin III, a urothelial marker, and α-SMA, a smooth muscle cell marker. These findings suggest that the ADSCs-SF scaffold not only supports the structural integrity of the bladder but also improves tissue regeneration and reduces adverse inflammatory responses, offering a promising approach for bladder repair and reconstruction.

使用脂肪干细胞种子蚕丝纤维支架增强膀胱再生:比较分析。
有效的膀胱重建仍然是泌尿外科的重大挑战,特别是对于需要部分或完全膀胱置换的情况。本研究评估了丝素蛋白(SF)和脂肪源性干细胞(ADSCs-SF)两种支架促进膀胱再生的效果及其相关结果。采用大鼠模型比较SF和ADSCs-SF支架移植膀胱组织的手术效果和形态恢复情况。评估术后恢复情况,包括排尿能力和并发症发生率。采用大体组织分析、组织计量学评估和免疫组织化学染色,在多个时间点(2、4、8和12周)评估再生膀胱组织的形态和组织学变化。两种支架类型均成功地融入膀胱壁,在体重或排尿问题上无显著差异。SF支架组出现移植物收缩,膀胱结石发生率为41.6%。相比而言,ADSCs-SF支架有助于更好的形态恢复,膀胱组织逐渐采用更正常的形状,没有膀胱结石的发生。组织学分析显示,ADSCs-SF支架显著促进了更有组织的尿路上皮层和平滑肌组织的再生。与单独的SF支架相比,它还导致更高的血管密度和减少炎症细胞的浸润。此外,ADSCs-SF组表现出关键标志物的表达增强,包括尿路上皮标志物uroplakin III和平滑肌细胞标志物α-SMA。这些结果表明,ADSCs-SF支架不仅支持膀胱的结构完整性,而且可以促进组织再生,减少不良炎症反应,为膀胱修复和重建提供了一种很有前景的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomimetics
Biomimetics Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
3.50
自引率
11.10%
发文量
189
审稿时长
11 weeks
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