SARS-CoV-2 Infection Among Children in Rondônia, Western Brazilian Amazon: SARS-CoV-2 Among Children in Rondônia.

IF 1.1 Q4 VIROLOGY
Advances in Virology Pub Date : 2025-02-17 eCollection Date: 2025-01-01 DOI:10.1155/av/6655790
Gil Guibson Mota Amaral, Gabriella Sgorlon, Valcimar Batista Ferreira, Flávia Serrano Batista, Luana da Silva Soares Farias, Luana Soares Barbagelata, Mirleide Cordeiro Dos Santos, Rayssa Layana da Silva Bedran, Deusilene Souza Vieira Dall'Acqua, Najla Benevides Matos
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引用次数: 0

Abstract

We analyzed 364 children symptomatic or asymptomatic for respiratory symptoms, aged 0.1 month-17 years, selected from primary healthcare units of different municipalities of Rondônia from June 2021 to September 2022. Data were collected from medical and electronic records for epidemiological characterization. The positive cohort (n = 96) was quantified using a real-time (RT) qPCR and sequenced by next-generation sequencing. Whole-genome sequences were obtained, SARS-CoV-2 strains were classified using the Pango system, and the maximum likelihood method was used for phylogenetic analyses. Among the patients, 59.34% (216/364) were male and 40.66% (148/364) were female. Children aged 10-14 years showed the highest rate of SARS-CoV-2 positivity. At the time of collection, 54.12% (197/364) of the patients were not age-eligible for immunization against COVID-19. The unvaccinated group accounted for 34.07% (124/364), with the highest proportion in the age groups of 5-9 and 10-14 years. Most patients exhibited mild symptoms. Seventy-nine high-quality genomes were obtained: Delta variant of concern (VOC) was the most prevalent (most abundant strain: AY.99.2), Omicron VOC was reported in 26 individuals (most frequent subvariant: BA.1.1), and Gamma VOC with 22 cases (12 cases of P.1 strain). The viral load showed a median of 7.26 log10 copies/mL, with a mean symptom duration of 4 days. Most of the cases were from children who were unvaccinated and age-ineligible for immunization and were associated with Delta and Omicron VOCs with an increase in subvariants during the study period.

巴西西部亚马逊Rondônia地区儿童SARS-CoV-2感染:Rondônia地区儿童SARS-CoV-2感染
我们分析了364名有呼吸道症状或无呼吸道症状的儿童,年龄0.1个月-17岁,他们来自Rondônia不同城市的初级卫生保健单位,时间为2021年6月至2022年9月。从医疗和电子记录中收集数据以进行流行病学特征分析。阳性队列(n = 96)采用实时荧光定量pcr (RT)定量,并采用下一代测序技术进行测序。获得全基因组序列,采用Pango系统对SARS-CoV-2菌株进行分类,并采用最大似然法进行系统发育分析。其中男性占59.34%(216/364),女性占40.66%(148/364)。10-14岁儿童的SARS-CoV-2阳性率最高。在收集时,54.12%(197/364)的患者不符合COVID-19免疫接种的年龄条件。未接种组占34.07%(124/364),以5-9岁和10-14岁年龄组比例最高。大多数患者表现出轻微的症状。获得79个高质量基因组,其中Delta变异(VOC)最为普遍(最丰富的菌株:AY.99.2), Omicron变异(最常见的亚变体:BA.1.1) 26例,Gamma VOC 22例(P.1菌株12例)。病毒载量中位数为7.26 log10拷贝/mL,平均症状持续时间为4天。大多数病例来自未接种疫苗和年龄不符合免疫条件的儿童,并且在研究期间与Delta和Omicron挥发性有机化合物的亚变体增加有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.30
自引率
0.00%
发文量
23
审稿时长
22 weeks
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