Advanced detection of bisphenol A in plastic water bottles using liquid–liquid phase extraction and LC-MS

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Vellaisamy Indirakumari, Dharmalingam Sakthilatha, Kumarasamy Jayakumar, Habibulla Imran, Sooman Lim and Mohammad Rashid Khan
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Abstract

The release of endocrine-disrupting chemicals (EDCs) can harm humans and wildlife. It is therefore important to monitor bisphenol A (BPA) consumption, an endocrine disruptor commonly found in water from plastic products, and detect BPA at low concentrations for accurate health risk assessments. We present a method for estimating BPA levels in plastic-bottled products that is highly sensitive, precise, and effective. BPA analysis was performed using advanced liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), with multiple reaction monitoring (MRM) on a state-of-the-art Orbitrap mass spectrometry system using negative ionization techniques. To assess the quality of Indian brands of water, we used LC-MS to obtain balanced hydrophilic–lipophilic extracts. Brand water samples showed efficiently separated BPA in 2.35 minutes, with other sources typically taking between 5 and 8 minutes. BPA concentrations, measured within a range of 10 ng mL−1 to 1 pg mL−1 with a lower detection limit (LOD) of 0.037 ng mL−1, were characterized by dynamic linear ranges and corresponding linear equations for each compound. We also evaluated the reproducibility and sensitivity of the detection of BPA in different water samples, including mineral, river, and tap water, with low levels of BPA found in Indian river water (below 4.54 ng mL−1). Thus, this study explored alternatives to solid phase extraction (SPE) for screening BPA analogs in water samples, and real samples from an Indian supermarket revealed BPA in plastic bottles at concentrations comparable to those described in Europe, the United States, Korea, Japan, and China.

Abstract Image

利用液相-液相萃取和液相色谱-质谱法对塑料水瓶中的双酚 A 进行高级检测。
内分泌干扰化学物质(EDCs)的释放会危害人类和野生动物。因此,重要的是监测双酚A (BPA)的摄入量,并检测低浓度的BPA,以准确评估健康风险。双酚A是一种内分泌干扰物,通常存在于塑料制品的水中。我们提出了一种估算塑料瓶装产品中BPA水平的方法,该方法高度敏感、精确和有效。双酚a分析采用先进的液相色谱-电喷雾串联质谱(LC-ESI-MS/MS),并在最先进的Orbitrap质谱系统上使用负电离技术进行多反应监测(MRM)。为了评估印度品牌水的质量,我们使用LC-MS获得平衡的亲水-亲脂提取物。品牌水样显示,有效分离BPA的时间为2.35分钟,而其他来源的水通常需要5到8分钟。BPA浓度测定范围为10 ng mL-1 ~ 1 pg mL-1,下限检出限(LOD)为0.037 ng mL-1,采用动态线性范围和相应的线性方程对各化合物进行表征。我们还评估了不同水样中BPA检测的再现性和灵敏度,包括矿物水、河水和自来水,在印度河水中发现了低水平的BPA(低于4.54 ng mL-1)。因此,本研究探索了固相萃取(SPE)在水样中筛选双酚a类似物的替代方法,并从印度超市的实际样品中发现塑料瓶中的双酚a浓度与欧洲、美国、韩国、日本和中国的浓度相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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