Moso bamboo sucrose synthase gene SUS3 promotes earlier germination of tiller buds in Dendrocalamus brandisii ‘Manxie Tianzhu’

{"title":"Moso bamboo sucrose synthase gene SUS3 promotes earlier germination of tiller buds in Dendrocalamus brandisii ‘Manxie Tianzhu’","authors":"Weijie Sun,&nbsp;Muthusamy Ramakrishnan,&nbsp;Long-Hai Zou,&nbsp;Kai-Li Wang,&nbsp;Xiaohong Zhou,&nbsp;Mingbing Zhou","doi":"10.1007/s00468-025-02612-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Key message</h3><p><b>Transient overexpression of </b><b><i>PeSUS3</i></b> <b>using a</b> <b><i>BaMV</i></b> <b>vector in</b><b><i> D. brandisii</i></b> <b>‘Manxie Tianzhu’ enhanced tillering bud germination and growth, suggesting a crucial role for sucrose metabolism in bamboo development.</b></p><h3>Abstract</h3><p>Bamboo shoots are highly sought after in China for their nutritional value. <i>Dendrocalamus brandisii</i> ‘Manxie Tianzhu’ (Manxie Sweet Dragon Bamboo) faces supply challenges due to limited distribution, necessitating faster growing, higher yielding varieties. The sucrose synthase gene, particularly <i>PeSUS3</i>, is vital for sucrose metabolism, nutrient transport, and regulating dormancy release and bud germination, yet its role in bamboo shoot production is not well understood. This study used a bamboo mosaic virus vector (BaMV) to overexpress <i>PeSUS3</i> in <i>D. brandisii</i> ‘Manxie Tianzhu’ seedlings. <i>PeSUS3</i> was cloned into the BaMV-P19-EGFP-2 vector, introduced into <i>Agrobacterium tumefaciens</i>, and used to infect tobacco leaves. The resulting virus was mechanically inoculated into the bamboo seedlings. Analysis revealed that <i>PeSUS3</i> overexpression increased the number of tiller buds and accelerated germination by 1–2 days compared to controls. Despite the transient overexpression of <i>PeSUS3</i>, the increased sucrose content in the overexpressing plants suggests a role for sucrose metabolism in these processes. These findings emphasize the importance of the transient overexpression of <i>PeSUS3</i> in promoting tillering bud germination and growth in <i>D. brandisii</i> ‘Manxie Tianzhu’, providing a foundation for future research on its function and mechanisms.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 2","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trees","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1007/s00468-025-02612-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FORESTRY","Score":null,"Total":0}