Metabolomic and genomic insights into Micromonospora carbonacea subsp. caeruleus for anti-colorectal compound

IF 3.9 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Microbiology and Biotechnology Pub Date : 2025-02-25 DOI:10.1007/s00253-025-13427-z

Tepakorn Kongsaya, Nuttaporn Emthomya, Chananan Ngamcharungchit, Aiyada Aroonsri, Umaporn Uawisetwathana, Thapanee Pruksatrakul, Jirayut Euanorasetr, Bungonsiri Intra

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Abstract

Cancer is a predominant contributor to global morbidity and mortality, affecting populations worldwide. Marine Micromonospora species have been identified as significant sources of anticancer compounds. This work aimed to perform a polyphasic approach of isolated strain and conduct comparative metabolomic and genomic analyses to identify compounds with anticancer activity. The study utilized a polyphasic approach on isolated strains for anticancer compound identification. Taxonomic analysis of strain 2MTK254 revealed unique pigment and fatty acid patterns, designating it as a novel Micromonospora carbonacea subsp. caeruleus. Its crude extract displayed significant anti-colorectal activity (66.03% inhibition). Molecular network analysis classified metabolites into eight classes, highlighting a polycyclic tetramate macrolactams (PTMs) compound (P1, C₂₉H₃₈N₂O₄) with 99.31% inhibitory activity against HCT-116 cell lines (IC₅₀ at 0.125 µM). Genome analysis identified 32 biosynthetic gene clusters (BGCs), including unique PTMs BGCs (83% similarity) linked to the P1 compound. Thus, M. carbonacea subsp. caeruleus 2MTK254 holds promise as a source of novel PTMs with anti-colorectal cancer potential.

• A novel strain of Micromonospora carbonacea subsp. caeruleus 2MTK254 was isolated in Thailand

• A new polycyclic tetramate macrolactam (PTM) with anticancer activity was identified in 2MTK254

• The genome of 2MTK254 has unique secondary metabolite gene clusters

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碳小单孢子菌亚种的代谢组学和基因组学研究。小檗为抗结直肠化合物

癌症是全球发病率和死亡率的主要原因，影响着全世界的人口。海洋小单孢子菌已被确定为抗癌化合物的重要来源。本工作旨在对分离菌株进行多相分析，并进行比较代谢组学和基因组学分析，以鉴定具有抗癌活性的化合物。本研究采用多相法对分离菌株进行抗癌化合物鉴定。对菌株2MTK254进行分类分析，发现该菌株具有独特的色素和脂肪酸结构，是一种新的碳小单孢子菌亚种。山雀。其粗提物具有显著的抗结直肠活性（抑制率为66.03%）。分子网络分析将代谢物分为8类，突出显示了多环四聚内酰胺（PTMs）化合物（P1, C29H38N2O4）对HCT-116细胞系的抑制活性为99.31% （IC50在0.125µM时）。基因组分析鉴定出32个生物合成基因簇（BGCs），包括与P1化合物相关的独特PTMs BGCs（相似度为83%）。因此，M. carbonacea亚sp。caeruleus 2MTK254有望成为具有抗结直肠癌潜力的新型ptm的来源。一株炭黑小单孢子菌亚种。•在2MTK254中发现一种新的具有抗癌活性的多环四聚内酰胺（PTM）•2MTK254基因组具有独特的次级代谢产物基因簇

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来源期刊

Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物

CiteScore

10.00

自引率

4.00%

发文量

535

审稿时长

2 months

期刊介绍： Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.