Fluorescent/colorimetric dual-mode for detecting of MC-LR using bidirectional RCA coupled with CdTe QDs

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-02-25 DOI:10.1007/s00604-025-06991-8

Weiqing Sun, Xinru Ren, Yijing Xiao, Bowen Li, Qing-ao Pang, Meili Yang, Rui Zhu, Zhiqiang Guo, Jinghua Yu, Jiadong Huang, Yu Wang, Su Liu

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Abstract

A fluorescent/colorimetric dual-mode biosensor was designed using CdTe QDs and CRISPR/Cas for the efficient and ultrasensitive detection of microcystin-leucine-arginine (MC-LR). The biosensor mainly activates the trans-cleavage activity of Cas12a through nucleic acid amplification technology, such as bidirectional rolling circle amplification (B-RCA), to perform signal conversion, release Ag⁺ from the cleaving hairpin, quench QD fluorescence, and perform signal presentation. The biosensor can perform fluorometric and colorimetric detection, enabling rapid field assays. It exhibits enhanced selectivity, increased sensitivity, and greater accuracy. The optimal conditions yield a detection range from 0.05 to 500 nM, with a minimum detectable concentration of 2.137 pM, surpassing the performance of traditional methods. The biosensor can effectively detect MC-LR in actual environmental samples. Overall, this study provided a general detection approach for the application of nucleic acid detection technology to detect trace pollutants in the environment.

Graphical Abstract

查看原文本刊更多论文

利用CdTe QDs和CRISPR/Cas设计了一种荧光/比色双模式生物传感器，用于高效、超灵敏地检测微囊藻毒素-亮氨酸-精氨酸（MC-LR）。该生物传感器主要通过双向滚圆扩增（B-RCA）等核酸扩增技术激活 Cas12a 的反向裂解活性，进行信号转换，释放裂解发夹中的 Ag+，淬灭 QD 荧光，进行信号呈现。该生物传感器可进行荧光和比色检测，实现快速现场检测。它具有更强的选择性、更高的灵敏度和更高的准确性。最佳条件下的检测范围为 0.05 至 500 nM，最低检测浓度为 2.137 pM，超越了传统方法的性能。该生物传感器能有效检测实际环境样品中的 MC-LR。总之，这项研究为应用核酸检测技术检测环境中的痕量污染物提供了一种通用的检测方法。图文摘要

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.