The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)

IF 1.1 3区 农林科学 Q3 ENTOMOLOGY
Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin
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Abstract

The silk of silkworm, Bombyx mori, consists mainly of proteins, which contain some small proteins with specialized functions that confer special properties to silk, such as antimicrobial properties. Serine protease inhibitors (SPIs) are one of them, and the types that have been identified are seroin, serpin, Kunitz type, and Kazal type, but the function of the Kunitz/Kazal type is not known. As compared to others, Kazal molecular weight is relatively minimal. In this study, we focused on the function of a Kazal-type inhibitor BmSPI2, and its sequence characterization and expression profile were first refined. To clarify its function, BmSPI2 recombinant protein was expressed in sf9 cells using baculovirus expression system. Further testing revealed that BmSPI2 could inhibit trypsin, α-chymotrypsin, and plasmin, whereas has only some effect on proteinase K. This suggests that BmSPI2 has significant serine protease inhibitory activity. In-depth analysis revealed that BmSPI2 also effectively inhibited fibrinogen degradation mediated by fibrinolytic enzymes. Finally, we examined its antimicrobial-related functions using prepared antibodies and found that BmSPI2 was also able to directly bind to bacteria and fungi to inhibit their proliferation. The results of the study enriched the function of BmSPI2 and laid a theoretical foundation for its development and utilization.

Abstract Image

家蚕kazal型丝氨酸蛋白酶抑制剂的功能研究
家蚕(Bombyx mori)的蚕丝主要由蛋白质组成,其中含有一些具有特殊功能的小蛋白质,这些蛋白质赋予蚕丝特殊的特性,如抗菌特性。丝氨酸蛋白酶抑制剂(SPIs)是其中之一,已鉴定的类型有血清素、丝氨酸蛋白酶抑制剂、Kunitz型和Kazal型,但Kunitz/Kazal型的功能尚不清楚。与其他植物相比,卡扎尔的分子量相对较小。在这项研究中,我们重点研究了kazal型抑制剂BmSPI2的功能,并首次完善了其序列表征和表达谱。为明确其功能,利用杆状病毒表达系统在sf9细胞中表达BmSPI2重组蛋白。进一步的实验表明,BmSPI2能抑制胰蛋白酶、α-凝乳胰蛋白酶和纤溶酶,而对蛋白酶k只有一定的抑制作用。这表明BmSPI2具有显著的丝氨酸蛋白酶抑制活性。深入分析发现,BmSPI2还能有效抑制由纤溶酶介导的纤维蛋白原降解。最后,我们利用制备的抗体检测了其抗菌相关功能,发现BmSPI2也能直接与细菌和真菌结合,抑制其增殖。研究结果丰富了BmSPI2的功能,为其开发利用奠定了理论基础。
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来源期刊
Journal of Asia-pacific Entomology
Journal of Asia-pacific Entomology Agricultural and Biological Sciences-Insect Science
CiteScore
2.70
自引率
6.70%
发文量
152
审稿时长
69 days
期刊介绍: The journal publishes original research papers, review articles and short communications in the basic and applied area concerning insects, mites or other arthropods and nematodes of economic importance in agriculture, forestry, industry, human and animal health, and natural resource and environment management, and is the official journal of the Korean Society of Applied Entomology and the Taiwan Entomological Society.
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