Overexpression of cytochrome P450 CYP71AF43 contributing resistance to fenoxaprop-P-ethyl in Alopecurus myosuroides from China

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2025-02-24 DOI:10.1016/j.pestbp.2025.106355

Junhui Tian , Dingyi Bai , Sifen He, Zongfang Li, Lianyang Bai, Lang Pan

{"title":"Overexpression of cytochrome P450 CYP71AF43 contributing resistance to fenoxaprop-P-ethyl in Alopecurus myosuroides from China","authors":"Junhui Tian , Dingyi Bai , Sifen He, Zongfang Li, Lianyang Bai, Lang Pan","doi":"10.1016/j.pestbp.2025.106355","DOIUrl":null,"url":null,"abstract":"<div><div>Black-grass (<em>Alopecurus myosuroides</em>), one of the most economically destructive herbicide-resistant weeds in Europe, is rapidly expanding in winter wheat regions of China. In recent years, the recommended application rate of fenoxaprop-P-ethyl in the field has failed to effectively control <em>Alopecurus myosuroides</em> populations, thereby threatening wheat yields at risk. In this study, we collected a suspected herbicide-resistant population (R-HB) of <em>Alopecurus myosuroides</em> from a wheat field in Hebei Province and confirmed its resistance to fenoxaprop-P-ethyl, with a resistance index of 26.73-fold. Sensitivity analyses of other ACCase-inhibiting herbicides revealed cross-resistance in the R-HB population to clethodim and pinoxaden. Molecular analysis indicated that the resistance phenotype in this population was not due to alterations in the target site. Pretreatment with the cytochrome P450 (P450) inhibitor malathion partially reversed fenoxaprop-P-ethyl resistance in the R-HB population. RNA-seq and RT-qPCR validation revealed the constitutive overexpression of the P450 gene <em>CYP71AF43</em> in the R-HB population. Molecular docking predictions suggest that the CYP71AF43 protein may have metabolic activity toward fenoxaprop-P-ethyl. In genetically modified yeast, overexpression of <em>AmCYP71AF43</em> was found to enhance tolerance to fenoxaprop-P-ethyl, but not to clethodim and pinoxaden. Additionally, rice calli overexpressing the <em>AmCYP71AF43</em> gene exhibited resistance to fenoxaprop-P-ethyl, but not to clethodim or pinoxaden. Collectively, the increased expression of <em>CYP71AF43</em> may enhance P450-mediated metabolism, conferring resistance to fenoxaprop-P-ethyl in the R-HB population. This is the first report of this mechanism in <em>Alopecurus myosuroides</em>. This discovery provides a novel perspective for the in-depth analysis of resistance mechanisms in weeds against the ACCase-inhibiting herbicide fenoxaprop-P-ethyl.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106355"},"PeriodicalIF":4.2000,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357525000689","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Black-grass (Alopecurus myosuroides), one of the most economically destructive herbicide-resistant weeds in Europe, is rapidly expanding in winter wheat regions of China. In recent years, the recommended application rate of fenoxaprop-P-ethyl in the field has failed to effectively control Alopecurus myosuroides populations, thereby threatening wheat yields at risk. In this study, we collected a suspected herbicide-resistant population (R-HB) of Alopecurus myosuroides from a wheat field in Hebei Province and confirmed its resistance to fenoxaprop-P-ethyl, with a resistance index of 26.73-fold. Sensitivity analyses of other ACCase-inhibiting herbicides revealed cross-resistance in the R-HB population to clethodim and pinoxaden. Molecular analysis indicated that the resistance phenotype in this population was not due to alterations in the target site. Pretreatment with the cytochrome P450 (P450) inhibitor malathion partially reversed fenoxaprop-P-ethyl resistance in the R-HB population. RNA-seq and RT-qPCR validation revealed the constitutive overexpression of the P450 gene CYP71AF43 in the R-HB population. Molecular docking predictions suggest that the CYP71AF43 protein may have metabolic activity toward fenoxaprop-P-ethyl. In genetically modified yeast, overexpression of AmCYP71AF43 was found to enhance tolerance to fenoxaprop-P-ethyl, but not to clethodim and pinoxaden. Additionally, rice calli overexpressing the AmCYP71AF43 gene exhibited resistance to fenoxaprop-P-ethyl, but not to clethodim or pinoxaden. Collectively, the increased expression of CYP71AF43 may enhance P450-mediated metabolism, conferring resistance to fenoxaprop-P-ethyl in the R-HB population. This is the first report of this mechanism in Alopecurus myosuroides. This discovery provides a novel perspective for the in-depth analysis of resistance mechanisms in weeds against the ACCase-inhibiting herbicide fenoxaprop-P-ethyl.

Abstract Image

查看原文本刊更多论文

细胞色素P450 CYP71AF43的过表达与对非诺沙丙- p-乙基的抗性

黑草（Alopecurus myosuroides）是欧洲最具经济破坏性的抗除草剂杂草之一，在中国冬小麦产区迅速蔓延。近年来，田间推荐用量的异诺沙丙-对乙基农药未能有效控制稻角霉种群，威胁小麦产量。本研究在河北省某麦田采集了一株疑似除草剂抗性种群（R-HB），证实其对异诺沙丙- p-乙酯具有抗性，抗性指数为26.73倍。对其他accase抑制除草剂的敏感性分析显示，R-HB群体对clethodim和pinoxaden具有交叉抗性。分子分析表明，该群体的抗性表型不是由于靶位点的改变。细胞色素P450 （P450）抑制剂马拉硫磷预处理部分逆转了R-HB人群对非诺沙丙- p-乙基的耐药性。RNA-seq和RT-qPCR验证显示P450基因CYP71AF43在R-HB人群中组成性过表达。分子对接预测表明CYP71AF43蛋白可能对fenoxapprop - p- ethyl有代谢活性。在转基因酵母中，AmCYP71AF43的过表达增强了对fenoxapprop - p- ethyl的耐受性，而对clethodim和pinoxaden则没有。此外，过量表达AmCYP71AF43基因的水稻愈伤组织对非诺沙丙烯- p-乙基具有抗性，但对氯噻虫胺和匹诺沙丁没有抗性。总的来说，CYP71AF43的表达增加可能会增强p450介导的代谢，在R-HB人群中赋予对fenoxapprop - p- ethyl的抗性。这是该机制在褐皮鸠中的首次报道。这一发现为深入分析杂草对accase抑制除草剂fenoxaprop-P-ethyl的抗性机制提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.