Evaluation of Paracentrotus lividus spines extract antioxidant, antidiabetic, anti-inflammatory, antimicrobial, and mechanistic anticancer: Insights into its composition using UPLC-ESI-MS-based metabolomic profiling

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-02-22 DOI:10.1016/j.tice.2025.102808

Maha M. Salem , Mai M. Elkeiy , Mona M. El-Gamal , Khalil M. Saad-Allah , Abeer A. Khamis

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引用次数: 0

Abstract

Sea urchins are widely distributed in waters worldwide. The present study carried out the in vitro metabolomic bioactivity profiling using UPLCMS/MS of Paracentrotus lividus spines extract. Investigations were also conducted on molecular anticancer, anti-inflammatory, antidiabetic, antioxidant, and antibacterial properties. A comprehensive untargeted metabolic profiling of P. lividus spines extract resulted in the classification of more than 13 metabolites. Their metabolomic quantitative evaluations were assumed by measuring total phenolic, flavonoids, dihydroflavonol, sugar, and protein contents. The P. lividus spines extract exhibited powerful antioxidant capacity using DPPH*, ABTS⁺, reducing power, and phosphomolybdate assays. Moreover, P. lividus spines extract highly elucidated antidiabetic and anti-inflammatory activity by inhibiting α-amylase enzyme and protein denaturation. Further, the spines of the P. lividus exhibited significant antibacterial effects. Besides, extract from P. lividus spines showed a strong cytotoxic impact against a variety of HepG-2 and MCF-7 cancer cell lines. It was discovered that the P. lividus spines extract triggered cell cycle arrest in the sub-G0/G1 phase and suppressed the growth of cancer cells via suppressing mRNA of Akt/MAPK/Bcl-2/c-myc and protein expression of β-Catenin/ki-67. Conclusively, the extract derived from the spines of the sea urchin species P. lividus demonstrates significant potential for utilization in various pharmaceutical industries.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.