Genomic surveillance and evolution of Getah virus.

IF 5.5 2区医学 Q1 VIROLOGY

Virus Evolution Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI:10.1093/ve/veaf007

Jiaqi Shen, Shaogui Liu, Shiqiang Liu, Shiyi Shen, Mingkai Lei, Qian Xu, Wentao Li, Qigai He, Xiaojuan Xu, Xuwang Cai

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Abstract

Getah virus (GETV), a member of the Alphaviruses, has spread widely and is expanding its host range worldwide, posing a serious threat to public health safety and the farming industry. However, genetic monitoring of GETV is inadequate, and its evolution and transmission remain unclear. This study employed reverse transcription-polymerase chain reaction to screen pig tissue samples for the presence of GETV. Subsequent steps included DNA sequencing, phylogenetic analysis, and selection pressure assessments to elucidate the evolutionary history and transmission patterns of the virus. A total of 1382 samples were examined, with a positivity rate of 4.12% (95% confidence interval: 3.07%-5.17%) from 2022 to 2023. Subsequently, seven GETV strains were isolated and identified. A phylogenetic tree was constructed, which showed that all seven strains belonged to Group III. Phylodynamic analysis revealed that GETV evolved rapidly. Additionally, eight amino acid sites within the GETV E2 protein were identified as being under positive selection. These data provide insight into the epidemiology and evolution of GETV.

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盖塔病毒的基因组监测和进化。

作为甲型病毒的一员，Getah病毒（GETV）在全球范围内广泛传播，并正在扩大其宿主范围，对公共卫生安全和农业构成严重威胁。然而，对GETV的遗传监测是不充分的，其进化和传播仍不清楚。本研究采用逆转录聚合酶链反应筛选猪组织样本中是否存在GETV。随后的步骤包括DNA测序、系统发育分析和选择压力评估，以阐明病毒的进化史和传播模式。共检测样本1382份，2022 - 2023年阳性率为4.12%（95%置信区间为3.07% ~ 5.17%）。分离鉴定了7株GETV菌株。经系统发育树分析，7株菌株均属于类群III。系统动力学分析表明，GETV进化迅速。此外，GETV E2蛋白中的8个氨基酸位点被鉴定为正选择。这些数据为了解GETV的流行病学和演变提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virus Evolution Immunology and Microbiology-Microbiology

CiteScore

10.50

自引率

5.70%

发文量

108

审稿时长

14 weeks

期刊介绍： Virus Evolution is a new Open Access journal focusing on the long-term evolution of viruses, viruses as a model system for studying evolutionary processes, viral molecular epidemiology and environmental virology. The aim of the journal is to provide a forum for original research papers, reviews, commentaries and a venue for in-depth discussion on the topics relevant to virus evolution.