Functional redundancy and divergence of UDP-glucose 4-epimerases in galactose metabolism and cell wall biosynthesis in Aspergillus nidulans

IF 2.3 3区生物学 Q3 GENETICS & HEREDITY

Fungal Genetics and Biology Pub Date : 2025-02-21 DOI:10.1016/j.fgb.2025.103972

Chihiro Kadooka , Shun Yakabe , Daisuke Hira , Taiki Futagami , Masatoshi Goto , Takuji Oka

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引用次数: 0

Abstract

Galactose-containing polysaccharides in the cell walls of filamentous fungi are vital for hyphal formation, mycelial aggregation, and adhesion. Uridine diphosphate (UDP)-glucose 4-epimerase, an enzyme capable of reversibly converting UDP-glucose to UDP-galactose, plays a key role in galactose metabolism. This study investigates the functional specialization and overlapping roles of UDP-glucose 4-epimerases, UgeA and UgeB, in Aspergillus nidulans. Enzyme activity assays revealed that UgeA catalyzes the interconversion of UDP-glucose and UDP-galactose, while UgeB facilitates both UDP-glucose/UDP-galactose and UDP-N-acetylglucosamine/UDP-N-acetylgalactosamine interconversions. Both UgeA and UgeB successfully restored growth in a yeast gal10 disruptant, indicating their involvement in galactose metabolism in vivo. Additionally, the ugeB disruptant of A. nidulans exhibited growth retardation during galactose metabolism, a defect that was alleviated by complementation with ugeB or multiple-copy expression of ugeA. These findings elucidate the complex interplay between sugar metabolism and cell wall synthesis in filamentous fungi and offer insights for the development of novel antifungal therapies.

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芽曲霉半乳糖代谢和细胞壁生物合成中udp -葡萄糖4-表戊酶的功能冗余和分化。

丝状真菌细胞壁中含半乳糖的多糖对菌丝的形成、菌丝的聚集和粘附至关重要。尿苷二磷酸(UDP)-葡萄糖4-甲酰基酶是一种能够将UDP-葡萄糖可逆转化为UDP-半乳糖的酶，在半乳糖代谢中起着关键作用。本研究探讨了UDP-glucose 4- epimease UgeA和UgeB在灰曲霉中的功能特化和重叠作用。酶活性分析表明，UgeA可以催化udp -葡萄糖和udp -半乳糖的相互转化，而UgeB可以促进udp -葡萄糖/ udp -半乳糖和udp - n -乙酰氨基葡萄糖/ udp - n -乙酰氨基半乳糖的相互转化。UgeA和UgeB都成功地恢复了酵母半乳糖干扰物的生长，表明它们参与了体内半乳糖代谢。此外，夹竹桃的ugeB干扰物在半乳糖代谢过程中表现出生长迟缓，这一缺陷可以通过与ugeB互补或ugeA的多拷贝表达来缓解。这些发现阐明了丝状真菌糖代谢和细胞壁合成之间的复杂相互作用，并为开发新的抗真菌疗法提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal Genetics and Biology 生物-遗传学

CiteScore

6.20

自引率

3.30%

发文量

审稿时长

85 days

期刊介绍： Fungal Genetics and Biology, formerly known as Experimental Mycology, publishes experimental investigations of fungi and their traditional allies that relate structure and function to growth, reproduction, morphogenesis, and differentiation. This journal especially welcomes studies of gene organization and expression and of developmental processes at the cellular, subcellular, and molecular levels. The journal also includes suitable experimental inquiries into fungal cytology, biochemistry, physiology, genetics, and phylogeny. Fungal Genetics and Biology publishes basic research conducted by mycologists, cell biologists, biochemists, geneticists, and molecular biologists. Research Areas include: • Biochemistry • Cytology • Developmental biology • Evolutionary biology • Genetics • Molecular biology • Phylogeny • Physiology.