Comparison of two fluorescence detectors in flow cytometry to predict malignant transformation of oral leukoplakia from cell cycle fractions.

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2025-02-10 DOI:10.1016/j.oooo.2025.01.726

Caroline Gennari Stevão, Matheus Henrique Lopes Dominguete, Paulo de Camargo Moraes, Victor Angelo Martins Montalli, André Luis Santana de Freitas, Marcelo Sperandio

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引用次数: 0

Abstract

Objective: The objective of this paper was to compare the accuracy of predicting malignant transformation of oral leukoplakia using the 585-620 nm detector when compared with data obtained with the 661-683 nm detector.

Study design: This was a single-factor retrospective longitudinal experimental study, whose factor will be DNA content (DNA ploidy) at 4 levels, G1 phase, S phase, mitosis phase (G2), and DNA excess (4cER). The sample is a cohort of individuals diagnosed with oral leukoplakia (N = 97) where 18 cases evolved into cancer and 79 did not.

Results: The DNA content parameters 4cER, G2, and G1/G2 ratios were associated with malignant transformation (P < .05). Two analog algorithms of these findings generated positive predictive values (PPVs) of 38% (67% sensitivity and 76% specificity) and 40% (61% sensitivity and 80% specificity). DNA content by flow cytometry can achieve a PPV of 40% in high-risk lesions and negative predictive value (NPV) of 90% in low-risk lesions.

Conclusions: There was no significant difference among the percentages of nuclei in the G1, S, G2, and excess G2 (4cER) phases comparing the 661-683 nm and 585-620 nm detectors. The 585-620 nm detector achieved PPV and NPV equivalent to those observed with the 661-683 nm detector.

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.