Identification by GWAS of marker haplotypes relevant to breed potato for Globodera pallida resistance.

IF 4.4 1区 农林科学 Q1 AGRONOMY
J Leuenberger, F Esnault, P L Lebas, S Fournet, M P Cann, S Marhadour, C Prodhomme, M L Pilet-Nayel, M C Kerlan
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引用次数: 0

Abstract

Key message: Identified and validated QTL GpaVa_MRQ and GpaIX_MRQ provide robust tools for improving potato resistance to Globodera pallida via marker-assisted selection. Potato (Solanum tuberosum L.), a vital food crop globally, faces significant yield losses due to potato cyst nematodes (PCN). This study aimed to identify and validate genomic regions conferring resistance to Globodera pallida, to facilitate the development of resistant potato varieties through marker-assisted selection (MAS). We conducted Genome-Wide Association Studies (GWAS) on a pre-breeding panel genotyped using Genotyping by Sequencing (GBS) and the SolCAP DNA array. Significant resistance-associated SNP markers were identified on chromosomes III, IV, V, IX and XI. Quantitative trait loci (QTL), including the major-effect QTL GpaVa_MRQ on chromosome V and the QTL GpaIX_MRQ on chromosome IX, were validated and shown to account for substantial phenotypic variance in a validation potato panel. Haplotype-based marker sets were defined at four QTL regions, enabling the practical application of MAS. The successful conversion of SNPs to PACE markers at the two main QTL GpaVa_MRQ and GpaIX_MRQ further supports their use in breeding programs. This study provides valuable insights and robust tools for enhancing potato resistance to G. pallida, contributing to sustainable agricultural practices and global food security.

通过 GWAS 鉴定与培育抗 Globodera pallida 马铃薯相关的标记单倍型。
鉴定和验证的QTL GpaVa_MRQ和GpaIX_MRQ为通过标记辅助选择提高马铃薯对白斑弧菌的抗性提供了强有力的工具。马铃薯(Solanum tuberosum L.)是全球重要的粮食作物,由于马铃薯囊肿线虫(PCN),马铃薯面临着重大的产量损失。本研究旨在鉴定和验证对苍白弧菌(Globodera pallida)具有抗性的基因组区域,以便通过标记辅助选择(marker assisted selection, MAS)培育马铃薯抗性品种。我们使用基因分型测序(GBS)和SolCAP DNA阵列对一个育种前群体进行了全基因组关联研究(GWAS)。在染色体III、IV、V、IX和XI上发现了显著的抗性相关SNP标记。数量性状位点(QTL),包括染色体V上的主要效应QTL GpaVa_MRQ和染色体IX上的QTL GpaIX_MRQ,被验证并证明在验证马铃薯面板中解释了大量表型差异。在4个QTL区域定义了基于单倍型的标记集,实现了MAS的实际应用。在两个主要QTL GpaVa_MRQ和GpaIX_MRQ上,snp成功转化为PACE标记,进一步支持了它们在育种计划中的应用。本研究为提高马铃薯对苍白弧菌的抗性提供了有价值的见解和强大的工具,有助于可持续农业实践和全球粮食安全。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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