Adhesion of Porphyromonas gingivalis and growth of human gingival fibroblasts on modified titanium dental implant surfaces exhibiting 1000-nm spikes.

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Quintessence international Pub Date : 2025-02-24 DOI:10.3290/j.qi.b5993849

Fariba Ashrafi, Monika Astasov-Frauenhoffer, Elizaveta Fasler-Kan, Sabrina Ruggiero, Roland Steiner, Laurent Marot, Fabien Sanchez, Sebastian Kühl, Michael M Bornstein, Khaled Mukaddam

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引用次数: 0

Abstract

Objectives: The aim of this study was to assess the antibacterial efficacy of nanostructured dental implant surfaces against Porphyromonas gingivalis, a crucial bacterium in the progression of peri-implantitis. Additionally, to evaluate the possible effect of the modified implant surface on the viability of human gingival fibroblasts (HGnFs).

Method and materials: As detailed in prior research, helium ions sputtering was used to produce nanospiked titanium surfaces with a height of 1000 nm (Ti1000). Smooth machined (Ti machined) and SLA titanium disks (TiSLA) served as controls. The antibacterial characteristics of the samples against P. gingivalis were evaluated by conventional culturing and SEM. The vitality of HGnFs was assessed using the MTT assay.

Results: Generally, nanostructured Ti1000 surfaces exhibited less bacterial counts than the two controls (Ti1000 vs TiSLA -36% and Ti1000 vs Ti machined -37%.) without being statistically significant. Moreover, the nanosurface did not affect the vitality of HGnFs.

Conclusion: The nanospikes of the modified titanium implant surface did not hinder gingival fibroblasts' vitality or proliferation characteristics. Optimizing the spacing and height parameters of the nanospikes could further improve the antibacterial effects of this unique re-modification approach.

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来源期刊

Quintessence international 医学-牙科与口腔外科

CiteScore

3.30

自引率

5.30%

发文量

审稿时长

1 months

期刊介绍： QI has a new contemporary design but continues its time-honored tradition of serving the needs of the general practitioner with clinically relevant articles that are scientifically based. Dr Eli Eliav and his editorial board are dedicated to practitioners worldwide through the presentation of high-level research, useful clinical procedures, and educational short case reports and clinical notes. Rigorous but timely manuscript review is the first order of business in their quest to publish a high-quality selection of articles in the multiple specialties and disciplines that encompass dentistry.