Urinary Metabolomic Profile is Minimally Impacted by Common Storage Conditions and Additives.

IF 1.8 3区医学 Q3 OBSTETRICS & GYNECOLOGY

International Urogynecology Journal Pub Date : 2025-02-24 DOI:10.1007/s00192-025-06069-2

Kelly C Weldon, Morgan Panitchpakdi, Andrés Mauricio Caraballo-Rodríguez, Alan J Wolfe, Pieter C Dorrestein, Linda Brubaker, Lindsey A Burnett

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引用次数: 0

Abstract

Background: Metabolomics reflects the molecular communications within biological systems. Urine is a noninvasive biofluid, rich in metabolites that serve as potential biomarkers for human health and disease. The impact of storage conditions and DNA stabilizers for urine samples in metabolomic studies remain unclear.

Objective: To evaluate the impact of common storage conditions and the presence of a DNA stabilizer, AssayAssure® (Thermo Scientific), on the metabolite content of voided human urine.

Methods: We assessed the urinary metabolite composition under different storage conditions and with the addition of AssayAssure® to determine its effect on metabolomic analysis.

Results: Urinary metabolite composition remained consistent across different storage conditions. However, the addition of AssayAssure® significantly altered the metabolic profile due to adduct formation. Despite these alterations, the identification of parent metabolites was not compromised, and biological differences were still distinguishable.

Conclusion: These findings suggest that urine biobanked under the tested storage conditions is suitable for metabolomic analysis. The addition of AssayAssure® does not hinder the detection of parent metabolites, although it may affect the overall metabolic profile.

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尿代谢组学概况是最小的影响，共同的储存条件和添加剂。

背景：代谢组学反映了生物系统内的分子通讯。尿液是一种无创生物流体，富含代谢物，可作为人类健康和疾病的潜在生物标志物。在代谢组学研究中，储存条件和DNA稳定剂对尿液样本的影响尚不清楚。目的：评估常见的储存条件和DNA稳定剂AssayAssure®（Thermo Scientific）的存在对人空尿代谢物含量的影响。方法：我们评估了不同储存条件下的尿液代谢物组成，并添加了AssayAssure®，以确定其对代谢组学分析的影响。结果：尿液代谢物组成在不同的储存条件下保持一致。然而，由于加合物的形成，AssayAssure®的加入显著改变了代谢谱。尽管有这些改变，鉴定亲本代谢物并没有受到损害，生物学差异仍然是可区分的。结论：在试验条件下建立的尿液生物库可用于代谢组学分析。添加AssayAssure®不会妨碍亲本代谢物的检测，尽管它可能会影响整体代谢谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Urogynecology Journal 医学-妇产科学

CiteScore

3.80

自引率

22.20%

发文量

406

审稿时长

3-6 weeks

期刊介绍： The International Urogynecology Journal is the official journal of the International Urogynecological Association (IUGA).The International Urogynecology Journal has evolved in response to a perceived need amongst the clinicians, scientists, and researchers active in the field of urogynecology and pelvic floor disorders. Gynecologists, urologists, physiotherapists, nurses and basic scientists require regular means of communication within this field of pelvic floor dysfunction to express new ideas and research, and to review clinical practice in the diagnosis and treatment of women with disorders of the pelvic floor. This Journal has adopted the peer review process for all original contributions and will maintain high standards with regard to the research published therein. The clinical approach to urogynecology and pelvic floor disorders will be emphasized with each issue containing clinically relevant material that will be immediately applicable for clinical medicine. This publication covers all aspects of the field in an interdisciplinary fashion