Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument.

Q3 Medicine

法医学杂志 Pub Date : 2024-10-25 DOI:10.12116/j.issn.1004-5619.2023.530601

Jun-Ping Han, Ying-Xi Wang, Chao Liu, Feng Liu, Jin-Rong Guo, Wei Zhu, Guang-Feng Tang, Cai-Xia Li, Xin Wang, Zhang-Ping Jiao

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Abstract

Objectives: To verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument (hereinafter referred to as Quick TargSeq DNA detection instrument) and evaluate its forensic application prospects.

Methods: A total of 74 blind samples provided by the expert group of Criminal Investigation Bureau of the Ministry of Public Security (4 individuals, 20 samples) and five participating institutions (54 samples) were used to test on 5 Quick TargSeq DNA detection instruments and the accompanying microfluidic chip cartridges. The results were compared with the known genotypes to calculate the samples' detection rate and genotyping accuracy.

Results: The results of reproducibility obtained from 20 samples provided by the expert group on the 5 Quick TargSeq DNA detection instruments were all consistent with the known genotypes. The detection rate of the 74 samples was 100%, and the genotyping accuracy was 97.33%.

Conclusions: The Quick TargSeq DNA detection instrument has good stability and high genotyping accuracy.

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