Xanthomonas campestris pv. campestris regulates virulence mechanisms by sensing blue light.

IF 2.7 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Photochemical & Photobiological Sciences Pub Date : 2025-02-01 Epub Date: 2025-02-24 DOI:10.1007/s43630-025-00694-y
V P Conforte, J Rinaldi, H R Bonomi, A Festa, D Garacoche, S Foscaldi, E Castagnaro, A A Vojnov, F Malamud
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引用次数: 0

Abstract

Light is an environmental stimulus to which all living organisms are exposed. Numerous studies have shown that bacteria can modulate virulence factors through photoreceptor proteins. Xanthomonas campestris pv. campestris (Xcc) is the causative agent of the systemic vascular disease black rot, which affects cruciferous crops worldwide. Typical symptoms include V-shaped yellow lesions emanating from the leaf margins and blackening of the leaf veins. In previous work, we have shown that Xcc possesses a functional bacteriophytochrome (XccBphP) that regulates its virulence in response to red and far-red light. In addition to the XccBphP protein the Xcc genome codes for a blue light photoreceptor, a Light Oxygen Voltage (LOV) domain-containing protein with a histidine kinase (HK) as the output module. Here, we show that both photoreceptors are able to sense blue light. We demonstrated that XccLOV is a functional photoreceptor by performing loss and gain of function experiments with a knock-out and a complemented strain for the lov gene. Blue light negatively affected swimming motility, whereas xanthan production was regulated by XccBphP, in a blue light independent manner. Additionally, our studies showed that blue light altered biofilm structure patterns and enhanced virulence. Overall, these results revealed that some Xcc virulence factors are blue light modulated via at least two photoreceptors.

油菜黄单胞菌。Campestris通过感应蓝光调节毒力机制。
光是一种环境刺激,所有生物都暴露在光下。大量研究表明,细菌可以通过光感受器蛋白调节毒力因子。油菜黄单胞菌。油菜属(campestris, Xcc)是引起十字花科作物系统性血管病黑腐病的病原菌。典型症状包括叶缘发出的v型黄色病变和叶脉变黑。在之前的工作中,我们已经证明Xcc具有功能性细菌色素(XccBphP),可以调节其对红光和远红光的毒力。除了XccBphP蛋白外,Xcc基因组还编码一种蓝光光感受器,一种含光氧电压(LOV)结构域的蛋白,其输出模块为组氨酸激酶(HK)。在这里,我们展示了两种光感受器都能够感知蓝光。通过对lov基因的敲除和补充菌株进行功能损失和增益实验,我们证明了XccLOV是一个功能性光感受器。蓝光对游泳运动有负面影响,而黄原胶的产生受XccBphP的调控,且与蓝光无关。此外,我们的研究表明,蓝光改变了生物膜的结构模式,增强了毒力。总的来说,这些结果揭示了一些Xcc毒力因子是通过至少两个光感受器来调节蓝光的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences 生物-生化与分子生物学
CiteScore
5.60
自引率
6.50%
发文量
201
审稿时长
2.3 months
期刊介绍: A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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