Study on Quality Markers of Tibetan Medicine Wuwei Shaji San Based on UHPLC-Q-Exactive Orbitrap-MS and Network Pharmacology Multicomponent Quantification

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-02-25 DOI:10.1002/bmc.70036

Yaozu Xiong, Chu Chen, Xiaohui Deng, Yanmei Hu, Haicui Liu, Li Wan, Leilei Du

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Abstract

The Tibetan medicine Wuwei Shaji powder (WWSJ) has been extensively applied to treating lung diseases such as chronic obstructive pulmonary disease (COPD). However, there are significant limitations in its quality control and evaluation. The purpose of this study was to analyze the overall chemical composition of WWSJ and to predict potential quality markers for WWSJ in the treatment of COPD. Using UHPLC-Q-Exactive Orbitrap-MS, 83 chemical constituents of WWSJ were identified or preliminarily characterized by 14 authentic reference standards, accurate mass counts, and characteristic fragment ions. In addition, serum pharmacology methods were employed to detect 13 blood-migrating components. Further network pharmacological research found that the potential mechanism of WWSJ in the treatment of COPD may be related to the regulation of the immune system and inflammatory response and identified 10 chemicals as key molecules in the treatment of COPD. Finally, the contents of six potential quality markers were determined simultaneously by high-performance liquid chromatography (HPLC). This study lays the foundation for further research on the mechanism of WWSJ in the treatment of COPD.

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基于uhplc - q -精确轨道谱联用及网络药理多组分定量的藏药五味沙棘散质量标记研究

藏药五味沙棘散被广泛应用于治疗慢性阻塞性肺疾病（COPD）等肺部疾病。然而，其质量控制和评价存在明显的局限性。本研究的目的是分析WWSJ的整体化学成分，并预测WWSJ治疗COPD的潜在质量指标。采用UHPLC-Q-Exactive Orbitrap-MS，通过14种标准品、准确的质量计数和特征片段离子，鉴定或初步表征了WWSJ的83种化学成分。此外，采用血清药理学方法检测13种血液迁移成分。进一步的网络药理学研究发现，WWSJ治疗COPD的潜在机制可能与调节免疫系统和炎症反应有关，并确定了10种化学物质作为治疗COPD的关键分子。最后，采用高效液相色谱法同时测定6种潜在质量标记物的含量。本研究为进一步研究WWSJ治疗COPD的作用机制奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.