Myelin Quantification Using Ultrashort Echo Time Magnetization Transfer Ratio in a Mouse Model of Traumatic Brain Injury

IF 2.3 4区医学 Q3 CLINICAL NEUROLOGY

Journal of Neuroimaging Pub Date : 2025-02-24 DOI:10.1111/jon.70029

Jiyo S. Athertya, Xin Cheng, Qingbo Tang, Dina Moazamian, Eric Y. Chang, Catherine E. Johnson, Jiankun Cui, Zezong Gu, Yajun Ma, Jiang Du

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引用次数: 0

Abstract

Background and Purpose

This study aims to assess the potential of ultrashort echo time imaging-based magnetization transfer ratio (UTE-MTR) in detecting demyelination in mice with mild traumatic brain injury (mTBI) caused by an open-field low-intensity blast (LIB) injury model.

Methods

This study included 30 male C57BL/6 mice, approximately 8 weeks old, sourced from Jackson Laboratories in Bar Harbor, ME, and conducted under institutional guidelines. The mice were divided into the mTBI group (n = 15) and the sham control group (n = 15). All animal experiments followed the approved protocols for the Care and Use of Laboratory Animals and Animal Research. The mTBI group underwent the open-field LIB injury. Behavioral tests were conducted to assess motor activity and anxiety-like responses. UTE-MT imaging was performed using a 3 Tesla Bruker system to measure UTE-MTR from two UTE-MT datasets with saturation powers of 1500° and 500°, and two frequency offsets of 2 and 50 kHz, respectively. Luxol fast blue (LFB) staining was performed to evaluate myelin content. The mean UTE-MTR values for regions of interest centered at the medial section of the corpus callosum were computed. The behavioral tests, LFB myelin staining, and UTE-MTR values were compared between the two groups using the independent t-test. p values <0.05 were considered significant.

Results

The mTBI mice demonstrated decreased motor activity and increased anxiety-like response over sham controls. The mTBI mice also showed significantly lower UTE-MTR values (0.399±0.007 vs. 0.393±0.005; p<0.05) and reduced LFB myelin staining (0.848±0.324 vs. 1.145±0.260; p = 0.048) over sham controls.

Conclusion

The significantly lower UTE-MTR values in the corpus callosum of mTBI mice are consistent with reduced LFB myelin staining, indicating that UTE-MTR can detect myelin loss and associated alterations in motor and anxiety domains post-LIB exposure.

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利用超短回波时间磁化传递比定量测定外伤性脑损伤小鼠模型中的髓磷脂

背景与目的本研究旨在探讨基于超短回波时间成像的磁化传递比（UTE-MTR）检测开放性低强度爆炸（LIB）损伤小鼠轻度创伤性脑损伤（mTBI）模型脱髓鞘的潜力。方法本研究采用30只雄性C57BL/6小鼠，约8周龄，来自Jackson实验室Bar Harbor， ME，并在机构指南下进行。将小鼠分为mTBI组（n = 15）和假对照组（n = 15）。所有动物实验均遵循经批准的实验动物和动物研究的护理和使用规程。mTBI组行开放性LIB损伤。进行行为测试以评估运动活动和焦虑样反应。使用3 Tesla Bruker系统对两个饱和功率为1500°和500°、频率偏移分别为2和50 kHz的UTE-MT数据集进行UTE-MT成像，测量UTE-MTR。Luxol快速蓝（LFB）染色测定髓磷脂含量。计算以胼胝体内侧为中心的感兴趣区域的平均UTE-MTR值。行为学测试、LFB髓磷脂染色、UTE-MTR值采用独立t检验比较两组间差异。P值<；0.05认为显著。结果与假对照相比，mTBI小鼠表现出运动活动减少和焦虑样反应增加。mTBI小鼠的UTE-MTR值也显著降低(0.399±0.007 vs 0.393±0.005；plt;0.05)， LFB髓磷脂染色降低(0.848±0.324∶1.145±0.260；P = 0.048)。结论mTBI小鼠胼胝体中显著降低的UTE-MTR值与LFB髓磷脂染色降低一致，表明UTE-MTR可以检测lib暴露后髓磷脂丢失及其相关的运动和焦虑区改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Neuroimaging 医学-核医学

CiteScore

4.70

自引率

0.00%

发文量

117

审稿时长

6-12 weeks

期刊介绍： Start reading the Journal of Neuroimaging to learn the latest neurological imaging techniques. The peer-reviewed research is written in a practical clinical context, giving you the information you need on: MRI CT Carotid Ultrasound and TCD SPECT PET Endovascular Surgical Neuroradiology Functional MRI Xenon CT and other new and upcoming neuroscientific modalities.The Journal of Neuroimaging addresses the full spectrum of human nervous system disease, including stroke, neoplasia, degenerating and demyelinating disease, epilepsy, tumors, lesions, infectious disease, cerebral vascular arterial diseases, toxic-metabolic disease, psychoses, dementias, heredo-familial disease, and trauma.Offering original research, review articles, case reports, neuroimaging CPCs, and evaluations of instruments and technology relevant to the nervous system, the Journal of Neuroimaging focuses on useful clinical developments and applications, tested techniques and interpretations, patient care, diagnostics, and therapeutics. Start reading today!