Metabolomics reveals the toxicity of polystyrene nanoplastics in the gills of Acrossocheilus yunnanensis

Abstract

Although the ecotoxicity of polystyrene nanoplastics (PS-NP) to fish has been widely reported, their impact on the metabolic processes in fish gills and the underlying mechanisms remains unclear. Here, we investigated the effects of PS-NP on the morphology, oxidative stress, and metabolism of Acrossocheilus yunnanensis gills using conventional physicochemical indicators and metabolomics analysis. The results showed that PS-NP caused oxidative stress, and resulted in gill tissue lesions (e.g., proliferation and sloughing of gill epithelial cells). Metabolomics results showed that PS-NP exposure induced 75–164 differentially expressed metabolites (DEMs) in fish gills, and they were mainly related to lipid metabolism. DEMs induced by high concentration of PS-NP compared with low concentration of PS-NP were not only significantly enriched in glycerophospholipid metabolism, but also in sphingolipid metabolism, nucleic acid metabolism, and a variety of signaling pathways. In conclusion, the results of this work suggest that PS-NP cause disruption of phospholipid metabolism mainly by disrupting the integrity of gill tissue, which provides a new perspective for understanding the impact mechanism of PS-NP on fish gills. Given that fish play essential roles in maintaining ecological balance, the adverse effects of PS-NP on fish gills could ultimately disrupt the stability and health of the aquatic ecosystem.