Exploring peroxidase mimetic activity of carbon nano-onions for colorimetric detection of H2O2 and glucose

IF 5.5 3区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of the Taiwan Institute of Chemical Engineers Pub Date : 2025-02-24 DOI:10.1016/j.jtice.2025.106029

Zahra Leili , Saeid Asadpour , Zeinab Saberi

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引用次数: 0

Abstract

Background

Developing sensing methods with nanozymes as alternatives to natural enzymes has gained significant attention from researchers.

Methods

In this paper, we demonstrated for the first time that carbon nano-onions (CNOs) act as a peroxidase-like mimetic activity towards the oxidation 3,3′,5,5′ tetramethylbenzidine (TMB) in association with hydrogen peroxide (H₂O₂) to produce a blue oxide TMB (oxTMB) with maximum absorption at 652 nm. Consequently, CNOs were utilized as a nanozyme for the colorimetric detection of H₂O₂ and glucose. The blue color of ox-TMB faded in the presence of glucose. The influence of various factors including acidity, temperature, time, CNOs and TMB concentrations were studied and optimized.

Significant findings

CNOs showed a good peroxidase-like mimetic activity. In this method, the concentration of H₂O₂ was detected in two linear ranges from 3 to 36 (nM) and from 36 to 450 (nM) with a detection limit (LOD) of 1 nM. Additionally, the results indicated that the absorption decreased with glucose concentrations ranging from 2.97 to 190 μM, achieving a LOD of 0.99 μM. The colorimetric possibility of this method was investigated in real plasma samples with recoveries of 95–103 %. Ultimately, the visual functionality of this colorimetric sensor can significantly improve and simplify the on-site detection process.

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来源期刊

Journal of the Taiwan Institute of Chemical Engineers 工程技术-工程：化工

CiteScore

9.10

自引率

14.00%

发文量

362

审稿时长

35 days

期刊介绍： Journal of the Taiwan Institute of Chemical Engineers (formerly known as Journal of the Chinese Institute of Chemical Engineers) publishes original works, from fundamental principles to practical applications, in the broad field of chemical engineering with special focus on three aspects: Chemical and Biomolecular Science and Technology, Energy and Environmental Science and Technology, and Materials Science and Technology. Authors should choose for their manuscript an appropriate aspect section and a few related classifications when submitting to the journal online.