pH-Responsive Triplex DNA Nanoswitches: Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs

IF 16 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-02-12 DOI:10.1021/acsnano.4c1639610.1021/acsnano.4c16396

Pei-Ying Lin, Ying-Feng Chang, Cheng-Che Chen, Li-Chen Su, Itamar Willner* and Ja-an Annie Ho*,

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Abstract

Bladder cancer (BC) has a high recurrence rate, necessitating frequent monitoring. We herein present an innovative method for detecting BC-related miR-183 and miR-155 microRNAs using pH-responsive triplex DNA nanoswitches (TDNs). This approach employs a stepwise surface plasmon resonance biosensing platform (TDNs-SPR assay) to detect these two miRNAs sequentially. The platform involves the assembly of two triplex pH-responsive probes, switch A (SA) and switch B (SB), on an SPR sensing interface by anchoring the probes to the surface through SA/miR-183 and SB/miR-155 binding to the S9.6 antibody-modified surface. The probes are functionalized with streptavidin-Au nanoparticles/biotinylated strands, which act as reporter units for the presence of the respective miRNAs on the sensing interface. The pH-induced displacement of reporter units triggers stepwise SPR reflectivity changes: at pH 5.0 for sensing miR-183 and at pH 8.3 for sensing miR-155. The reflectivity changes relate quantitatively to the concentrations of miRNAs. This sensing platform enables the detection of two miRNAs with detection limits as low as 0.57 pM for miR-183 and 0.83 pM for miR-155, highlighting its powerful utility for precise biomarker analysis. Moreover, this platform distinguishes BC patients from healthy individuals in urine samples. The method offers a versatile, noninvasive method for detecting any two miRNAs associated with other diseases.

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ph响应的三重DNA纳米开关：膀胱癌相关微小rna的表面等离子体共振平台

膀胱癌（BC）复发率高，需要经常监测。我们在此提出了一种创新的方法来检测bc相关的miR-183和miR-155 microrna，使用ph响应的三重DNA纳米开关（TDNs）。该方法采用逐级表面等离子体共振生物传感平台（TDNs-SPR测定）依次检测这两种mirna。该平台包括在SPR传感接口上组装两个三重ph响应探针，开关A （SA）和开关B (SB)，通过SA/miR-183和SB/miR-155结合到S9.6抗体修饰的表面，将探针锚定在表面。探针被链亲和素-金纳米颗粒/生物素化链功能化，它们作为传感界面上各自mirna存在的报告单元。pH诱导的报告单元位移触发SPR反射率的逐步变化：在pH 5.0时检测miR-183，在pH 8.3时检测miR-155。反射率的变化与mirna的浓度有关。该传感平台能够检测两种mirna， miR-183的检测限低至0.57 pM， miR-155的检测限低至0.83 pM，突出了其在精确生物标志物分析方面的强大功能。此外，该平台通过尿液样本将BC患者与健康个体区分开来。该方法为检测与其他疾病相关的任何两种mirna提供了一种通用的、无创的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.