Bacillus thuringiensis-derived pesticidal proteins toxic to the whitefly, Bemisia tabaci

Abstract

The whitefly, Bemisia tabaci, is among the most important threats to global agriculture and food security. In addition to losses associated with feeding, B. tabaci vectors hundreds of plant viruses, many of which cause severe disease in staple food crops. The management of B. tabaci is confounded by extensive resistance to chemical insecticides. While pesticidal proteins derived from entomopathogenic bacteria such as Bacillus thuringiensis (Bt) could provide for alternative management approaches, only one pesticidal protein with toxicity to B. tabaci has been identified. Here we screened 11 Bt-derived pesticidal proteins from several different structural classes against the highly invasive, Middle East-Asian Minor 1 (MEAM1) cryptic species of B. tabaci, and assessed the impact of a B. tabaci-active protein on the gut epithelial membrane by transmission electron microscopy. The pesticidal proteins were expressed in Bt or in Escherichia coli and purified for use in bioassays. The toxicity of purified proteins was first assessed by feeding adults on a single dose followed by lethal concentration (LC₅₀) determination for proteins with significant mortality relative to the buffer control. The proteins Tpp78Aa1, Tpp78Ba1, and Cry1Ca were toxic to B. tabaci with LC₅₀ values of 99, 96, and 351 µg/mL, respectively. Disruption of the brush border and severe reduction in microvilli on the gut surface caused by Tpp78Aa1 is consistent with the mode of action of Bt-derived pesticidal proteins. These proteins may provide valuable tools for the integrated management of B. tabaci populations and associated reduced incidence of B. tabaci vectored plant viral diseases.