Recycling slaughterhouse waste rumen fluid for biodegradable bioplastic production from Bacillus tequilensis KU 844284.

IF 2.2 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Sundaram Meignanalakshmi, Radhakrishnan Legadevi
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引用次数: 0

Abstract

In the present study, bioplastic - producing Bacillus tequilensis, a gram-positive rod, was isolated from slaughterhouse waste rumen fluid. Polyhydroxy butyrate (PHB) was produced from B. tequilensis using nitrogen-deficient Minimal medium (NDMM) and slaughterhouse waste rumen fluid (SHWRF). B. tequilensis produced PHB 4.51 ± 0.06 g/L and 1.60 ± 0.04 g/L from SHWRF and NDMM medium, respectively. Thermal stability, biodegradability, and biocompatibility were among the desirable properties of the PHB that were confirmed by FTIR, XRD, TGA, and DSC analysis. The PHB produced by using the SHWRF medium was degraded at a rate of 60.30% in 45 days. The in vitro cytotoxicity assay using the 3T3-L1 fibroblast cell line demonstrated that it is not harmful. This is the first report of bioplastic production by B.tequilensis using slaughterhouse waste rumen fluid. Rumen fluid from slaughterhouse waste can be recycled to produce biodegradable bioplastic.

本研究从屠宰场废弃瘤胃液中分离出了可产生生物塑料的茶枯草芽孢杆菌(一种革兰氏阳性杆菌)。利用缺氮最低限度培养基(NDMM)和屠宰场废弃瘤胃液(SHWRF)从茶碱芽孢杆菌中生产出了聚羟基丁酸(PHB)。在 SHWRF 和 NDMM 培养基中,B. tequilensis 产生的 PHB 分别为 4.51 ± 0.06 g/L 和 1.60 ± 0.04 g/L。傅立叶变换红外光谱、X射线衍射、热重分析和 DSC 分析证实了 PHB 的热稳定性、生物降解性和生物相容性等理想特性。使用 SHWRF 培养基生产的 PHB 在 45 天内降解了 60.30%。使用 3T3-L1 成纤维细胞系进行的体外细胞毒性试验表明,它对人体无害。这是 B.tequilensis 利用屠宰场废弃物瘤胃液生产生物塑料的首次报道。屠宰场废弃物中的瘤胃液可以循环利用,生产可生物降解的生物塑料。
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来源期刊
Environmental Technology
Environmental Technology 环境科学-环境科学
CiteScore
6.50
自引率
3.60%
发文量
0
审稿时长
4 months
期刊介绍: Environmental Technology is a leading journal for the rapid publication of science and technology papers on a wide range of topics in applied environmental studies, from environmental engineering to environmental biotechnology, the circular economy, municipal and industrial wastewater management, drinking-water treatment, air- and water-pollution control, solid-waste management, industrial hygiene and associated technologies. Environmental Technology is intended to provide rapid publication of new developments in environmental technology. The journal has an international readership with a broad scientific base. Contributions will be accepted from scientists and engineers in industry, government and universities. Accepted manuscripts are generally published within four months. Please note that Environmental Technology does not publish any review papers unless for a specified special issue which is decided by the Editor. Please do submit your review papers to our sister journal Environmental Technology Reviews at http://www.tandfonline.com/toc/tetr20/current
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