{"title":"Recycling slaughterhouse waste rumen fluid for biodegradable bioplastic production from <i>Bacillus tequilensis</i> KU 844284.","authors":"Sundaram Meignanalakshmi, Radhakrishnan Legadevi","doi":"10.1080/09593330.2025.2462794","DOIUrl":null,"url":null,"abstract":"<p><p>In the present study, bioplastic - producing <i>Bacillus tequilensis</i>, a gram-positive rod, was isolated from slaughterhouse waste rumen fluid. Polyhydroxy butyrate (PHB) was produced from <i>B. tequilensis</i> using nitrogen-deficient Minimal medium (NDMM) and slaughterhouse waste rumen fluid (SHWRF). <i>B. tequilensis</i> produced PHB 4.51 ± 0.06 g/L and 1.60 ± 0.04 g/L from SHWRF and NDMM medium, respectively. Thermal stability, biodegradability, and biocompatibility were among the desirable properties of the PHB that were confirmed by FTIR, XRD, TGA, and DSC analysis. The PHB produced by using the SHWRF medium was degraded at a rate of 60.30% in 45 days. The in vitro cytotoxicity assay using the 3T3-L1 fibroblast cell line demonstrated that it is not harmful. This is the first report of bioplastic production by <i>B.tequilensis</i> using slaughterhouse waste rumen fluid. Rumen fluid from slaughterhouse waste can be recycled to produce biodegradable bioplastic.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-16"},"PeriodicalIF":2.2000,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Technology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1080/09593330.2025.2462794","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
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Abstract
In the present study, bioplastic - producing Bacillus tequilensis, a gram-positive rod, was isolated from slaughterhouse waste rumen fluid. Polyhydroxy butyrate (PHB) was produced from B. tequilensis using nitrogen-deficient Minimal medium (NDMM) and slaughterhouse waste rumen fluid (SHWRF). B. tequilensis produced PHB 4.51 ± 0.06 g/L and 1.60 ± 0.04 g/L from SHWRF and NDMM medium, respectively. Thermal stability, biodegradability, and biocompatibility were among the desirable properties of the PHB that were confirmed by FTIR, XRD, TGA, and DSC analysis. The PHB produced by using the SHWRF medium was degraded at a rate of 60.30% in 45 days. The in vitro cytotoxicity assay using the 3T3-L1 fibroblast cell line demonstrated that it is not harmful. This is the first report of bioplastic production by B.tequilensis using slaughterhouse waste rumen fluid. Rumen fluid from slaughterhouse waste can be recycled to produce biodegradable bioplastic.
期刊介绍：
Environmental Technology is a leading journal for the rapid publication of science and technology papers on a wide range of topics in applied environmental studies, from environmental engineering to environmental biotechnology, the circular economy, municipal and industrial wastewater management, drinking-water treatment, air- and water-pollution control, solid-waste management, industrial hygiene and associated technologies.
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