Identification of Bacteria Isolated From Leaves of American Beech, Fagus grandifolia, Infested by the Foliar Nematode, Litylenchus crenatae (Nematoda: Anguinoidea), in New York, USA

IF 1.3 4区 农林科学 Q3 FORESTRY
Forest Pathology Pub Date : 2025-02-24 DOI:10.1111/efp.70009
Sebastian Albu, Irina P. Starodumova, Lubov V. Dorofeeva, Lyudmila I. Evtushenko, Sergei A. Subbotin
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引用次数: 0

Abstract

American beech (Fagus grandifolia) trees exhibiting symptoms of beech leaf disease (BLD) induced by the foliar nematode, Litylenchus crenatae, were found growing in Delmar, New York, USA, in 2019. Foliar symptoms included leaf drop, galling, and darkening associated with leaf curl. Bacterial isolates were cultured from infected leaf tissue and identified to genus by 16S rRNA gene sequencing and MALDI-TOF-MS profiling. In addition to many taxa with putatively environmental roles, strains of the genera Agrobacterium, Curtobacterium, Pantoea, Xanthomonas, as well as Erwinia and Pseudomonas were detected. Strains of the latter two genera have been previously associated with BLD, but isolates have not yet been identified at the species level. Phylogenetic trees generated from sequences of 16S rRNA and housekeeping genes (gyrB, rpoB, and rpoD) combined with MALDI-TOF-MS, showed that the isolates from nematode-infected leaves of American beech belonged to the known plant pathogenic species C. flaccumfaciens, E. billingiae, E. rhapontici, Pa. agglomerans, and X. arboricola. The species affiliation of strains showing close relatedness to A. skierniewicense, A. vaccinii, Pa. alfalfae, Pa. ananatis, Ps. amygdali, Ps. cerasi, and Ps. syringae requires further support. The identities of other bacteria isolated from the same samples are also provided.

美国纽约美洲山毛榉叶面线虫,Litylenchus crenatae(线虫纲:蛇尾总科)的分离细菌鉴定
2019年,在美国纽约州德尔马(Delmar)发现了由叶面线虫Litylenchus crenatae引起的山毛榉叶病(BLD)症状。叶片症状包括与叶片卷曲相关的落叶、刺痛和变黑。通过16S rRNA基因测序和MALDI-TOF-MS图谱鉴定,从感染的叶片组织中分离出细菌属。除了许多假定具有环境作用的分类群外,还检测到农杆菌属、Curtobacterium属、Pantoea属、Xanthomonas属以及Erwinia属和Pseudomonas属的菌株。后两属的菌株以前与BLD有关,但尚未在物种水平上鉴定分离株。利用16S rRNA序列和整理基因(gyrB、rpoB和rpoD)结合MALDI-TOF-MS构建的系统发育树显示,从线虫感染的美洲山毛榉叶片中分离的菌株属于已知的植物病原物种C. flaccumfaciens, E. billingiae, E. rhapontici, Pa。团聚,和X.树。与牛痘伊蚊、牛痘伊蚊、宾州伊蚊亲缘关系比较。alfalfae, Pa。黄芪、扁桃、黄芪和丁香需要进一步的支持。还提供了从同一样品中分离出的其他细菌的特性。
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来源期刊
Forest Pathology
Forest Pathology 农林科学-林学
CiteScore
2.70
自引率
0.00%
发文量
62
审稿时长
12-24 weeks
期刊介绍: This peer reviewed, highly specialized journal covers forest pathological problems occurring in any part of the world. Research and review articles, short communications and book reviews are addressed to the professional, working with forest tree diseases caused by fungi, bacteria, nematodes, viruses, and phytoplasms; their biology, morphology, and pathology; disorders arising from genetic anomalies and physical or chemical factors in the environment. Articles are published in English. Fields of interest: Forest pathology, effects of air pollution and adverse environmental conditions on trees and forest ecosystems.
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