Titelbild: Entschlüsselung der Polymer-Interaktionen in Biokonjugaten unterschiedlicher Architektur durch zeitaufgelöste limitierte Proteolyse mit massenspektrometrischer Analyse (Angew. Chem. 9/2025)

Angewandte Chemie Pub Date : 2025-02-10 DOI:10.1002/ange.202502486

Dr. Prisca Hamm, Dr. Marc D. Driessen, Dr. Niklas Hauptstein, Dr. Josef Kehrein, Rafael Worschech, Dr. Paria Pouyan, Prof. Dr. Rainer Haag, Prof. Dr. Ulrich S. Schubert, Prof. Dr. Thomas D. Müller, Prof. Dr. Dr. Lorenz Meinel, Prof. Dr. Tessa Lühmann

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Abstract

Limited proteolysis mass spectrometry allows for in deep molecular characterization of complex bioconjugates. In their Communication (e202415354), Lorenz Meinel, Tessa Lühmann et al. present a protocol for time-resolved proteolysis, deciphering interaction sites within protein–protein complexes and protein–polymer bioconjugates by ¹⁵N labeling. The cover image showcases the proteolysis of interferon-α2a and interferon-bioconjugates, resulting in peptide fragments that are analyzed using mass spectrometry.

Abstract Image