Control of Pseudomonas fluorescens in milk by a novel bacteriophage YZU_PF006

IF 2.8 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

International Journal of Dairy Technology Pub Date : 2024-10-02 DOI:10.1111/1471-0307.13135

Lei Yuan, Xinhai Yuan, Caowei Chen, Wenyuan Zhou, Zhenbo Xu, Thanapop Soteyome, Zhenquan Yang

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Abstract

Pseudomonas fluorescens is a psychrophilic bacterium that can cause dairy spoilage through the production of heat-stable enzymes. Bacteriophages are proved as one of the alternatives to control spoilage bacteria in the dairy industry. In this work, a lytic bacteriophage YZU_PF006 against P. fluorescens was isolated from sewage samples in China. Morphology and one-step growth results indicated that this short-tailed phage (a tail length of 5 nm and a head diameter of 58 nm) had a high burst size (180 PFU/infected cells) but a relatively short latent period (60 min). This phage can be inactive after pasteurisation and Clean-In-Place (CIP) procedures. The genomic sequence showed that phage YZU_PF006 had 47 open reading frames (ORFs) but did not have genes for virulence, lysogeny and antibiotic resistance. Phage YZU_PF006 effectively controlled P. fluorescens growth at 7°C and 28°C in milk, which indicates potential for the control P. fluorescens in the dairy industry.

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新型噬菌体YZU_PF006对牛奶中荧光假单胞菌的控制

荧光假单胞菌是一种嗜冷细菌，可以通过产生热稳定酶导致乳制品变质。在乳制品工业中，噬菌体已被证明是控制腐败菌的替代方法之一。本研究从中国污水样品中分离到一株抗荧光假单胞菌的噬菌体YZU_PF006。形态学和一步生长结果表明，该短尾噬菌体（尾长5 nm，头直径58 nm）具有高爆发大小（180 PFU/感染细胞），但潜伏期较短（60 min）。这种噬菌体在经过巴氏消毒和就地清洁（CIP）程序后可以失去活性。基因组测序结果显示，噬菌体YZU_PF006有47个开放阅读框（orf），但不含毒力、溶原性和耐药基因。噬菌体YZU_PF006在牛奶中7°C和28°C条件下均能有效控制荧光假单胞菌的生长，显示了在乳制品行业中控制荧光假单胞菌的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Dairy Technology 工程技术-食品科技

CiteScore

7.00

自引率

4.50%

发文量

审稿时长

12 months

期刊介绍： The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope. Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.